Galectin-3蛋白表达与胃癌腹膜转移关系的研究  

Study on association between the expression of galectin 3 and the peritoneal metastasis in gastric cancer

作　　者：杨志明[1] 伍晓汀[1] 何涛[1] 达明绪[1] 罗婷[1] 钱昆[1] 

机构地区：[1]四川大学华西医院普通外科,成都610041

出　　处：《中华胃肠外科杂志》2005年第2期151-154,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：国家教育部重点项目基金资助(02056)

摘　　要：目的探讨胃癌组织中半乳糖结合蛋白(galectin)-3蛋白表达与胃癌腹膜转移的关系。方法应用鼠抗人galectin-3单克隆抗体和免疫组织化学SP染色法检测35例同期伴腹膜转移胃癌患者的癌旁正常胃黏膜、胃癌原发灶、胃癌腹膜转移灶及淋巴结转移灶中galectin-3蛋白的表达。结果胃癌原发灶、胃癌腹膜转移灶及淋巴结转移灶中galectin-3强表达,且阳性表达率为97%(34/35);胃癌旁正常胃黏膜中galectin-3蛋白表达很弱,阳性表达率为14%(5/35)。正常胃黏膜中galectin-3的表达与胃癌原发灶、腹膜转移灶及淋巴结转移灶之间比较,差异具统计学意义(P<0.05)。胃癌原发灶、腹膜转移灶及淋巴结转移灶3者间的表达差异无统计学意义(P>0.05)。结论胃癌病灶中的galectin-3检查可能成为判断胃癌术前是否腹膜转移及预后的生物学指标。Objective To investigate the association between the expression of galectin 3 protein and peritoneal metastasis in gastric cancer. Methods The expressions of galectin 3 was detected in matching samples including primary gastric cancer lesions,lymph node metastases,peritoneal metastases and paratumor normal tissues by immunohistochemistry. All specimens were gained from 35 patients who had synchronous peritoneal metastasis from gastric cancer. Results The over expression of galectin 3 was observed in 97%(34/35) of the gastric cancer lesions,the peritoneal metastases and the lymph node metastases,whereas in 14%(5/35) of paratumor normal tissues. There were significant differences in the expression of galectin 3 between paratumor normal tissues and the gastric carcinoma lesions,peritoneal metastases and lymph node metastases (P< 0.05),but there were no significant differences among the gastric cancer lesions,the peritoneal metastases,and the lymph node metastases (P >0.05). Conclusion The expression of galectin 3 in gastric cancer lesions can be used as a biological marker of peritoneal metastasis from gastric cancer before operation and as a prognostic factor of gastric cancer.

关 键 词：胃癌 GALECTIN-3 腹膜转移 原发灶 淋巴结转移灶 蛋白表达 正常 结合蛋白 表达差异 染色法 

分 类 号：R735.2[医药卫生—肿瘤] R736.1[医药卫生—临床医学]