低剂量辐射对荷瘤鼠放疗后血清中抗氧化酶活性的影响  

Effects of Low Dose Radiation on Antioxidant Enzymes after Radiotherapy of Tumor-bearing Mice

作　　者：李进[1] 高刚[1] 王芹[1] 唐卫生[1] 刘晓秋[1] 王知权[1] 

机构地区：[1]中国医学科学院放射医学研究所,天津300192

出　　处：《中国辐射卫生》2005年第1期6-7,共2页Chinese Journal of Radiological Health

基　　金：天津市自然科学基金资助 (0 2 361 1 1 1 1 )

摘　　要：目的　观察低剂量辐射对荷瘤鼠放疗后血清中抗氧化酶活性的影响。方法　采用化学比色法测定不同照射时相点血清中超氧化物歧化酶 (SOD)、谷胱甘肽 -S转移酶 (GST)和过氧化氢酶 (CAT)活性。结果　低剂量全身照射组血清中SOD、GST、CAT活力高于相应对照组 ;低剂量 +局部放疗组 (d3d5)血清中SOD、GST、CAT活力显著高于局部大剂量组 (c3,c5) ;尤以连续三天照射组明显。Objective To search for effects of low dose radiation on the activities of antioxidant enzymes after radiotherapy of tumor-bearing mice. Methods Superoxide dismutase (SOD), glutathione-S-transferase(GST) and catalase(CAT) were all determined by chemical colorimetry. Results Low dose radiation increase the activities of antioxidant enzymes superoxide dismutase (SOD), glutathione-S-transferase(GST) and catalase (CAT) in serum of tumor-bearing mice more markedly than those in the unirradiated controls. The activities of antioxidant enzymes SOD, GST, CAT in serum of tumor-bearing mice (d 5, d 3) irradiated with 5cGy 6h before 2.0Gy radiation are obviously higher than those of the group(c 3, c 5) given with radiotherapy only. Conclusion The increase in the activities of antioxidant enzymes in serum of tumor-bearing mice triggered by low dose radiation could partly contribute to the protective mechanism.

关 键 词：抗氧化物酶 肿瘤 小鼠 动物实验 放射治疗 酶活性 照射剂量 荷瘤机体 

分 类 号：R730.55[医药卫生—肿瘤]