Apo2.7抗体原位标记法在原发性肝癌病人外周血淋巴细胞凋亡检测中的应用价值  

Evaluation of labeled Apo2.7 antibody in detecting in situ the apoptotic PBLC of patients with HCC.

作　　者：宋卫青[1] 吕维红[1] 陈华波[1] 辛永宁[1] 

机构地区：[1]青岛大学医学院附属青岛市市立医院,青岛266011

出　　处：《华中医学杂志》2005年第1期7-8,共2页Central China Medical Journal

基　　金：山东省卫生厅青年科技基金资助课题(No.2001CA2BBDA1)

摘　　要：目的　探讨Apo2.7抗体原位检测细胞凋亡的应用价值。方法　采集42例原发性肝癌病人和37例健康人的 外周血,分离外周血淋巴细胞后,分别采用Apo2.7抗体原位检测法和流式细胞仪检测细胞凋亡,并将结果进行比较。结果　 原发性肝癌病人Apo2.7抗体原位检测外周血淋巴细胞凋亡率为(12.4±1.2)%,流式细胞仪检测细胞凋亡率为(12.1± 0.7)%,2种方法检测外周血淋巴细胞凋亡率无显著性差异(t=1.43,P>0.05),但均明显高于对照组(P<0.01)。结论　 Apo2.7抗体原位检测法可替代流式细胞仪用于检测外周血淋巴细胞的凋亡;原发性肝癌病人淋巴细胞凋亡率高于正常人。Objective To explore the value of Apo 2.7 monoclonal antibody in detecting in situ the apoptotic peripheral blood lymphocytic cells (PBLC) of patients with HCC.Methods Whole blood of 42 patients with HCC and 37 healthy controls were collected,and PBLC were separated. The Apoptotic PBLC of all samples were detected by using both flow cytometric detection method and method of labeled Apo 2.7 antibody detection in situ.Results There was significant difference in the apoptotic rate of PBLCs between the two methods (t= 1.43 ,P> 0.05 ). The apoptotic rate of PBLCs by method of labeled Apo 2.7 antibody detection in situ was ( 12.4 ± 1.2 ) %, while that by flow cytometric detection method was ( 12.1 ± 0.7 ) %, both were significantly higher than that in controls ( P< 0.01 ).Conclusion The method of labeled Apo 2.7 antibody detection in situ can detect the apoptotic PBLC as a substitute for flow cytometric detection method. Lymphocyte apoptotic rate in the patients with HCC was higher than in the controls.

关 键 词：APO2.7 原发性肝癌 病人 抗体 原位检测 外周血淋巴细胞凋亡 流式细胞仪检测 人外周血淋巴细胞 采集 健康人 

分 类 号：R735.7[医药卫生—肿瘤]