组胺和低氧对猪肺动脉和主动脉内皮细胞eNOS基因表达的影响  

Effects of histamine and hypoxia on eNOS gene expression in cultured porcine pulmonary and aorta endothelial cells

作　　者：高景霞[1] 叶红[1] 李会革[1] 王迪浔[1] 

机构地区：[1]华中科技大学同济医学院病理生理系,卫生部呼吸系疾病重点实验室,湖北武汉430030

出　　处：《中国病理生理杂志》2005年第3期459-464,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .39990 0 5 5 )

摘　　要：目的 :观察组胺和低氧对培养的猪肺动脉和主动脉内皮细胞eNOSmRNA和蛋白质表达的影响。方法 :采用半定量RT -PCR和免疫细胞化学的方法。结果 :(1)组胺可使肺动脉内皮细胞eNOSmRNA表达增加 ,在10 -5mol/L作用 2 4h达高峰 ,为对照组的 178 2 %± 7 7% (P <0 0 1) ,eNOS蛋白质表达也上调 ,为对照组的 173%±4 7% (P <0 0 1) ,主动脉内皮细胞与肺动脉内皮细胞相似 ,可在组胺 10 -6mol/L作用 2 4h达高峰 ,为对照组的 177 4 %± 14 2 % (P <0 0 1) ,eNOS蛋白质表达也上调 ,为对照组的 16 5 %± 5 4 % (P <0 0 1) ;(2 )急性低氧 12h肺动脉内皮细胞eNOSmRNA表达增加 ,2 4h达高峰 ,为对照组的 15 1 0 %± 9 1% (P <0 0 1) ;蛋白质表达水平也增高到常氧对照组的 2 16 %± 4 4 % (P <0 0 1) ,而主动脉内皮细胞eNOSmRNA与蛋白质均未受低氧影响。结论 :组胺可使肺动脉和主动脉内皮细胞eNOS表达增加 ,但两者反应无明显差异 ;低氧使肺动脉内皮细胞eNOS表达上调 ,而对主动脉内皮细胞无明显影响。AIM: To investigate the effect of histamine and hypoxia on the expression of eNOS mRNA and protein in cultured porcine pulmonary artery and aorta endothelial cells. METHODS: Semi-quantitative RT-PCR and immuno-cytochemistry were used. RESULTS: (1) Histamine increased eNOS mRNA expression in a dose-and time dependent manner. For pulmonary endothelial cells, the effect reached peak when exposed to 10 -5 mol/L histamine in 24 h. eNOS mRNA level was increased to 178 2%±7 7% ( P <0 01) compared with control. eNOS protein was also enhanced to 173%±47% ( P <0 01) compared with control. For aorta endothelial cells, the effect reach peak when exposed to 10 -6 mol/L histamine in 24 h. The eNOS mRNA level was increased to 177 4%±14 3% ( P <0 01) compared with control. The eNOS protein was also enhanced to 165%±54% ( P <0 01). (2) The eNOS mRNA was enhanced in pulmonary endothelial cells after exposed to hypoxia for 12 h and reached peak in 24 h, increasing to 151 0%±9 1% ( P <0 01). The protein expression was also enhanced to 216%±44% ( P <0 01) compared with control. But there was no significant change in eNOS mRNA and protein expression in aorta endothelial cells during hypoxia. CONCLUSION: The experiments show that histamine increases the endothelial eNOS expression in both pulmonary and aorta endothelial cells, whereas hypoxia only increases eNOS expression in pulmonary endothelial cells. This may account partly for the different responses of pulmonary circulation and systemic circulation to hypoxia.

关 键 词：组胺 缺氧 一氧化氮合酶 基因表达调控 

分 类 号：R363[医药卫生—病理学]