佐剂关节炎大鼠脊神经节中核因子kappa B表达的研究  

Expression of nuclear factor kappa B in the spinal ganglions of adjuvant arthritis rats

作　　者：沈晖[1] 鲁静[1] 肖卫国[1] 方秀斌[2] 

机构地区：[1]中国医科大学附属第一医院风湿免疫科 [2]中国医科大学神经生物学教研室,辽宁沈阳110001

出　　处：《中国病理生理杂志》2005年第3期494-496,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助 (No.30 0 70 330 ) ;辽宁省教育厅高等学校科学研究基金资助项目 (No .2 0 12 10 32 )

摘　　要：目的 :研究核因子kappaB(NF -κB)在佐剂关节炎 (AA)大鼠脊神经节 (L1-L4)中的表达 ,以探讨类风湿关节炎 (RA)发病的神经 -内分泌—免疫学机制。方法 :用免疫组织化学法检测脊神经节中NF -κB蛋白表达 ,用Westernblotting法分析脊神经节细胞胞核蛋白中NF -κB含量改变。结果 :在AA大鼠L1-L4脊神经节细胞中 ,NF-κB蛋白表达明显高于正常对照组 (P <0 0 1) ,且活化的NF -κB(胞核蛋白中NF -κB)含量明显高于正常对照组(P <0 0 1)。细胞核内P6 5蛋白表达量与关节肿胀程度呈正相关。结论 :脊神经节中NF -κB活化可能参与RA发病机制。AIM: To study the expression of nuclear factor kappa B (NF-κB) in the L 1-L 4 spinal ganglions of adjuvant arthritis (AA) rats, to explore the neuroendocrinoimmunological mechanism of rheumatoid arthritis (RA). METHODS: The expression of the NF-κB protein was detected by immunohistochemistry method and the alteration of the active NF-κB protein (nuclear protein) was investigated by Western blotting analysis. RESULTS: The expression of NF-κB protein in L 1-L 4 spinal ganglions of AA rats was much higher than that in the healthy controls ( P <0 01) , and the active NF-κB content in L 1-L 4 spinal ganglions in AA rats was much higher than that in the healthy controls ( P <0 01). CONCLUSION: The active NF-κB protein in the spinal ganglions may be involved in the pathogenesis of the rheumatoid arthritis.

关 键 词：关节炎 类风湿 关节炎 实验性 脊神经 NF—κB 

分 类 号：R363[医药卫生—病理学]