四逆汤抗心肌缺血作用的相关蛋白谱研究  

Proteomic study on the anti-myocardial ischemia mechanisms of Sini decoction

作　　者：吴伟康[1] 李劲平[2] 罗汉川[1] 许传超[1] 

机构地区：[1]中山大学中西医结合研究所,广东广州510089 [2]中南大学药学院,湖南长沙410078

出　　处：《中国病理生理杂志》2005年第3期506-510,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助课题 (No .39770 936 )

摘　　要：目的 :探讨四逆汤保护缺血心肌的相关蛋白改变谱。方法 :利用二维凝胶电泳分离左心室肌总蛋白 ,通过PDQuest7 1 1软件分析 2 -DE图谱 ,比较各组间蛋白的表达量 ,然后利用MALDI -TOF -MS制作差异蛋白点的肽谱确定各差异蛋白的种类 ,通过生物信息学分析所得蛋白的功能。结果 :四逆汤调节缺血心肌的能量代谢、信号转导、机能、心肌细胞修复和抗氧自由基损伤等多组相关蛋白的表达 ,对缺血心肌产生保护作用。结论 :四逆汤具有良好的保护缺血心肌作用。AIM: To investigate the mechanisms of the anti-myocardial ischemia of Sini decoction at the level of proteome. METHODS: The total proteins of left ventricular muscle were separated with two-dimensional electrophoresis. The 2-DE images were analyzed with the PDQuest7 1 1 software package. The peptide mass fingerprinting(PMF) was made with MALDI-TOF-MS, then the identification and function of the protein was made via bioinformatics. RESULTS: Sini decoction could regulate the expression of 13 proteins in the ischemic myocardium. The proteins could allocated into five groups: energy metabolism-associated proteins, signal-associated proteins ,self-repair-associated proteins, anti-free radical injury-associated proteins and myocardial contractile-associated proteins. CONCLUSION: Sini decoction could prevent the myocardium from ischemia via regulating expression of different proteins.

关 键 词：四逆汤 中草药 心肌缺血 蛋白质组 

分 类 号：R363[医药卫生—病理学]