白藜芦醇诱导人胃癌原代细胞裸鼠移植瘤凋亡的研究  

Apoptosis of implanted tumor of human primary gastric cancer cells in nude mice induced by resveratrol

作　　者：周海波[1] 蔡建庭[1] 李茂岚[1] 汪竹峰 

机构地区：[1]浙江大学附属二院消化科,浙江杭州310009 [2]南浔中西医结合医院消化科,浙江南浔313000

出　　处：《中国病理生理杂志》2005年第3期528-532,共5页Chinese Journal of Pathophysiology

基　　金：浙江省青年人才专项基金资助项目(2 0 0 4QN0B)

摘　　要：目的 :探讨白藜芦醇诱导人胃癌原代细胞裸鼠移植瘤凋亡的机制。方法 :建立人胃癌原代细胞裸鼠移植瘤模型 ,采用瘤旁注射的方法 ,用不同剂量的白藜芦醇进行治疗 ,并设对照 ,用透射电镜和TUNEL法检测肿瘤组织细胞凋亡情况 ,免疫组织化学法和RT -PCR法检测肿瘤组织的凋亡相关基因bcl- 2和bax的表达情况。结果 :白藜芦醇对胃癌原代细胞移植瘤有明显的抑制作用 ;透射电镜和TUNEL法发现白藜芦醇导致移植瘤内大量细胞发生凋亡 ;免疫组织化学法发现白藜芦醇注射组的Bcl- 2蛋白阳性细胞率 (3 92 %± 0 85 %、5 70 %± 0 84 %和 11 86 %±0 97% )明显低于两个对照组 (P <0 0 5 ) ,两个对照组 (2 7 5 6 %± 1 4 0 %和 2 9 4 8%± 0 5 1% )之间无显著差异 ;Bax蛋白阳性细胞率 (5 2 30 %± 1 5 4 %、4 5 72 %± 0 88%和 4 0 0 2 %± 1 2 0 % )明显高于两个对照组 (P <0 0 5 ) ,两个对照组(2 0 88%± 0 91%和 19 34%± 0 35 % )之间无显著差异 ;RT -PCR法发现白藜芦醇注射组的bcl- 2mRNA条带强度随剂量的增大而递减 ,并明显低于两个对照组 (P <0 0 5 ) ,两个对照组之间无显著差异 (P >0 0 5 ) ;baxmRNA条带强度递增 ,并明显高于两个对照组 (P <0 0 5 ) ,两个对照组之间无显著差异 (P >0 0 5 )。AIM: To investigate the molecular mechanism of the apoptosis of implanted tumor of human primary gastric cancer cells in nude mice induced by resveratrol. METHODS: Human primary gastric cancer cells were planted into nude mice to establish the cancer model. Resveratrol at different doses were injected near the carcinoma on the nude mice. After treatment, transmission electron microscope and TUNEL staining method were used to detect the apoptosis of implanted tumor cells. Immunohistochemical staining and RT-PCR were used to detect the expression of apoptosis-related genes bcl-2 and bax in implanted tumor. RESULTS: Resveratrol significantly inhibited carcinoma growth when it was injected near the carcinoma. The apoptotic cells in implanted tumor induced by resveratrol were detected by transmission electron microscope and TUNEL staining, immunohistochemical staining and RT-PCR showed resveratrol inhibited bcl-2 expression and increased bax expression in human primary gastric cancer cells. CONCLUSION: Resveratrol inhibits implanted tumor of human primary gastric cancer cells in nude mice through inducing apoptosis. This apoptosis may be mediated by down-regulation of bcl-2 expression and up-regulation of bax expression.

关 键 词：白藜芦醇 胃肿瘤 小鼠 裸 细胞凋亡 

分 类 号：R735[医药卫生—肿瘤]