原发性肝细胞肝癌血小板源性生长因子的表达和肿瘤血管形成关系的相关研究  

Study on the relationship between expression of platelet-derived growth factor and microvessel density in hepatocellular carcinoma

作　　者：齐庆安[1] 别平[1] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所,400038

出　　处：《消化外科》2005年第2期119-123,共5页Journal of Digestive Surgery

摘　　要：　目的　初步探讨肝细胞性肝癌组织中血小板源性生长因子(platelet derived growthfactor,PDGF)及血管内皮细胞生长因子(vascular endothelial cell growth factor,VEGF)的表达与微血管密度(micro vessel density,MVD)的表达之间的关系。方法　对51 例肝癌组织及癌旁非肿瘤组织,20例正常肝组织,采用免疫组化(SABC法)检测PDGF、VEGF和CD34的表达。采用ELISA法,检测48例术前肝癌患者和16例肝内胆管结石患者血清中PDGF的浓度。结果　PDGF、VEGF在肝细胞癌组织中的阳性表达率分别为54.9%、64.7%,在癌旁非肿瘤组织中分别为 27.5%、37.3%,在正常肝组织中分别为5%、10%,三者之间比较差异有显著性意义(P<0.01),PDGF、VEGF阳性表达率与肝细胞癌的转移(P=0.031,P=0.04)、包膜形成(P=0.035, P=0.016)以及细胞分化程度(P=0.02,P=0.034)有关;与性别、年龄、肿瘤的大小没有关联。肝癌组织、癌旁组织和正常肝组织的MVD值分别是29.43±4.818、15.23±2.77(P<0.01)、14.56±1.96(P<0.01)。术前肝癌患者和肝内胆管结石患者血清中PDGF的浓度分别为458.33±303.17、125.00±92.69,二组之间比较有显著差异(P<0.05)。结论　PDGF在肝癌的发生,发展和浸润过程中可能起着重要促生长和转移作用,并且和VEGF可能存在一定的协同作用。Objective To investigate the relationships between the expression of PDGF, VEGF and MVD in HCC. Methods Immunohistochemical method was used to detect the expression of CD34, PDGF and VEGF in cancerous, paracarcinoma tissues and 20 cases of normal liver tissues. ELISA was used to detect serum PDGF concentration in HCC and cholecyst calculus patients. ResultsThe positive expression of PDGF, VEGF was 54.9%, 64.7% in HCC, 27.5%, 37.3% in paracarcinoma tissues, 5%, 10% in normal liver tissues. There were significantly differences among the three groups. Both PDGF and VEGF expression were related to capsule formation, metastasis, tumor differentiation degree, but not to gender, age, tumor size. The average MVD value of the three groups was 29.43±4.818, 15.23±2.77 (P<0.01), 14.56±1.96 (P<0.01) respectively. Preoperation serum PDGF concentration HCC and cholecyst calculus patients was 458.33±303.17 and 125.00±92.69. There was significant difference between the two groups (t=3.303, P= 0.005). ConclusionsPDGF may play an important role in the development and metastasis of HCC. Meanwhile, as a regulator vascular endothelial cell growth factor, PDGF may have cooperative action with VEGF.

关 键 词：肝细胞肝癌 微血管密度 血小板源性生长因子 血管内皮生长因子 

分 类 号：R735.7[医药卫生—肿瘤]