应用肿瘤基因解剖工程数据库检测大肠癌基因表达谱  

Detection of the profile of gene expression in colon cancer by means of cancer gene anatomy project and RT-PCR

作　　者：黄志刚[1] 冉志华[2] 陈锦先[3] 陆玮[1] 萧树东[2] 

机构地区：[1]复旦大学医学院附属华山医院消化内科,上海200040 [2]上海市消化疾病研究所上海第二医科大学附属仁济医院消化内科 [3]上海市消化疾病研究所上海第二医科大学附属仁济医院外科

出　　处：《中华消化杂志》2005年第2期79-82,共4页Chinese Journal of Digestion

摘　　要：目的进一步了解大肠癌与正常组织间基因表达谱差异,寻找可能用于临床诊断和治疗的基因标志.方法应用肿瘤基因解剖工程(CGAP)数据库中基因表达短序列分析(SAGE)数据筛查大肠癌和正常组织差异表达基因,并用RT-PCR方法进一步在大肠癌细胞株(SW1116、Lovo、HCT-8、Hce-8693)和20例组织标本中验证.结果在SAGE数据库中共分析大肠癌和正常组织短序列195 160个,发现差异基因216个.对其中17个基因进行RT-PCR验证分析,在细胞株中基因检出阳性率为35.3%～76.5%,组织标本中阳性率为88.2%.对其中转化生长因子β1、蛋白酶体26S亚单位、热休克蛋白1进行半定量RT-PCR,基因差异表达率分别为60%、50%、35%.结论利用CGAP数据库中SAGE数据能快捷、可靠地筛查大肠癌差异基因表达谱,对这些差异基因进一步分析可能为临床诊治大肠癌提供基因标志.Objective To investigate the differential gene expression profiles between colorectal tumors and normal tissues for further identifying gene markers that may be useful in the diagnosis and treatment of colorectal tumors. Methods The data of serial analysis of gene expression (SAGE) (provided) (by cancer) gene anatomy project (CGAP) were employed to analyze differential transcripts from normal and cancerous colonic epithelium. These transcripts were further confirmed by RT-PCR analysis in colon cancer cell lines (SW1116, Lovo, HCT-8 and Hce-8693) and 20 specimens of colorectal cancer. Results A total of 195 160 tags from normal and cancerous tissue were analyzed in SAGE data base , 216 were found to be differential genes. Seventeen transcripts were further confirmed by RT-PCR with a positive rate ranging from 35.3% to 76.5% in the cell lines and (88.2%) in the specimens. The differential transcriptions of transforming growth factor β1,PSMC4 and HSPCA were identified in 60%, 50% and 35% of specimens, respectively. Conclusions The differential gene expression profiles of colorectal cancer could be analyzed rapidly and reliably by means of the data of SAGE from CGAP. Further investigation on these transcripts may provide the gene markers for the detection and treatment of colorectal tumors.

关 键 词：大肠癌 正常组织 肿瘤基因 癌基因表达 组织标本 筛查 解剖 SAGE 差异基因 蛋白酶体 

分 类 号：R735.34[医药卫生—肿瘤] R730[医药卫生—临床医学]