趋化因子在实验性急性胰腺炎早期发病机制中的作用  

The role of cytokine-induced neutrophil chemoattractant and monocyte chemoattractant protein-1/JE in the pathogenesis of early acute pancreatitis

作　　者：许永春[1] 李兆申[1] 屠振兴[1] 龚燕芳[1] 施新岗[1] 汪良芝[1] 满晓华[1] 金晶[1] 

机构地区：[1]第二军医大学长海医院消化科,上海200433

出　　处：《中华消化杂志》2005年第2期90-94,共5页Chinese Journal of Digestion

摘　　要：目的　探讨中性粒细胞趋化因子(CINC)和单核细胞趋化蛋白(MCP-1/JE)在急性胰腺炎(AP)早期发病机制中的作用。方法　分别以浓度为0.5%和5%的牛磺胆酸钠逆行胆胰管注射制备大鼠急性水肿型胰腺炎(AEP)和急性坏死型胰腺炎(ANP)模型,同时设假手术对照组,检测血清淀粉酶、生化指标、观察胰腺组织湿干重比、髓过氧化物酶(MPO)活性和病理学改变,检测胰腺组织中 CINC及MCP-1/JE基因和蛋白表达。结果　与假手术对照组阴性表达相比,造模各组胰腺腺泡细胞内均有强弱不等的CINC和MCP-1/JE蛋白表达,胰腺组织MPO活性和CINC mRNA、MCP-1/JE mRNA表达均随AP逐渐加重及时间进展呈不断上调趋势(P< 0. 05 或 P< 0. 01),且 CINC mRNA、MCP-1/JEmRNA的表达都与胰腺组织病理学改变呈正相关。结论　AP 发生早期,胰腺腺泡细胞为 CINC 和MCP-1/JE的来源之一,在AP早期发病机制中发挥重要作用。Objective To explore the potential role of cytokine-induced neutrophil chemoattractant (CINC) and monocyte chemoattractant protein-1/JE (MCP-1/JE) in the pathogenesis of early acute pancreatitis(AP). Methods Acute edematous pancreatitis(AEP) and acute necrotizing pancreatitis(ANP) were induced by retrograde infusion of 0.5% or 5% sodium taurocholate into the biliary pancreatic duct. The activity of serum amylase and the level of serum calcium were studied, wet to weight ratio,the activity of myeloperoxidase (MPO) and pathological changes of pancreas were observed. The expression of gene and protein of CINC and MCP-1/JE in pancreatic constitution were detected. Results Compared with that in controls,pancreatic acinar cells were found to express CINC and MCP-1/JE protein in certain intensity. With the induction and the progress of AP,the expression of the activity of MPO,CINC and MCP-1/JE gene in pancreatic parenchyma were all significantly increased ( P <0.05, P <0.01). The (expression) of CINC and MCP-1/JE gene significantly correlated with the pathological changes in (pan-)(creatic) parenchyma.Conclusions Acinar cells may be a source of CINC and MCP-1/JE in early AP. They might play important roles in the pathogenesis of AP.

关 键 词：MCP-1 CINC 早期 胰腺组织 发病机制 急性胰腺炎 趋化因子 RNA 胰腺腺泡细胞 基因 

分 类 号：R576[医药卫生—消化系统]