核糖体S6激酶与大鼠肝纤维化相关性研究  

The role of ribosomal S6 kinase in the pathogenesis of rat hepatic fibrosis

作　　者：杨妙芳[1] 张新 强晖[1] 孙田美[1] 谢渭芬[1] 

机构地区：[1]第二军医大学长征医院消化内科,上海200003 [2]国家人类基因组南方研究中心

出　　处：《中华消化杂志》2005年第2期98-100,共3页Chinese Journal of Digestion

基　　金：国家自然科学基金资助项目(30270604);全军医药卫生科研基金资助项目(01MA153)

摘　　要：目的　探讨核糖体S6激酶(RSK)在大鼠肝纤维化发生中的作用。方法　采用腹腔注射二甲基亚硝胺的方法构建大鼠实验性肝纤维化模型。以免疫荧光双标记 激光扫描共聚焦显微镜成像技术检测RSK与α-平滑肌肌动蛋白(α- SMA)及Ⅰ型胶原。进一步利用免疫组化法检测 RSK与Ⅰ、Ⅲ型胶原在肝纤维化组织中的表达及其相关性。结果　在纤维化肝组织中,RSK的定位与α-SMA完全一致,Ⅰ型胶原伴随RSK周围分布。RSK与Ⅰ、Ⅲ型胶原的表达量之间亦呈显著相关(P<0.05)。结论　肝纤维化发生中,RSK定位于活化的肝星状细胞内,并参与胶原的表达调控。RSK可能成为治疗肝纤维化的新靶点。Objective To investigate the role of ribosomal S6 kinase (RSK) in the pathogenesis of rat hepatic fibrosis. Methods Intra-abdominal injection of dimethylnitrosamine was carried out to (establish) the model of rat experimental hepatic fibrosis. Immunofluorescent double labeling laser scanning (confocal) microscope was performed to detect the location of RSK, α-smooth muscle actin(α-SMA) and the collagen type Ⅰ. Immunohistochemistry was used to determine the correlation of the expression of RSK and collagen type Ⅰ, Ⅲ in the tissue of hepatic fibrosis. Results In the tissue of hepatic fibrosis, RSK was coexpressed with α-SMA, and the collagen type Ⅰ located around RSK. The expression of RSK was also correlated with that of collagen type Ⅰ and Ⅲ( P<(0.05) ). Conclusions In the development of (hepatic) fibrosis, RSK was mainly located in the activated hepatic stellate cell (HSC), and (involved) in (regulating) the expression of collagen in HSC. RSK is likely to be a new target for the (treatment) of hepatic (fibrosis.)

关 键 词：肝纤维化 大鼠 鼠肝 Α-SMA Ⅲ型胶原 Ⅰ型胶原 核糖体 激酶 表达调控 表达量 

分 类 号：R575.2[医药卫生—消化系统]