MUC1核心肽多克隆抗体的制备及鉴定  

Preparation and Identification of Polyclonal Antibody Against MUC1 Core Peptide

作　　者：唐艳[1] 李慧[2] 张培因[2] 卫红飞[2] 王莉[2] 李大鹏[2] 于永利[2] 王丽颖[2] 

机构地区：[1]吉林大学第二医院肿瘤内科,长春130041 [2]吉林大学基础医学院,长春130021

出　　处：《中国生物制品学杂志》2005年第2期135-137,共3页Chinese Journal of Biologicals

摘　　要：目的　制备MUC1核心肽多克隆抗体 ,用于靶向MUC1肿瘤治疗的研究。方法　以 8R -MUC1核心肽 6重复序列重组蛋白 (8R MUCPT)为抗原免疫家兔 ,以ELISA法确定抗MUC1多克隆抗体效价 ,以Westernblot和ELISA阻断试验鉴定抗体特异性。结果　MUC1核心肽多克隆抗体效价为 1∶2 5 6 0 0 0 ,能与MUC1核心肽特异性结合。结论　利用原核表达的 8R -MUCPT蛋白 ,可有效地诱导免疫动物产生MUC1核心肽特异性抗体 ,为MUC1核心肽靶向肿瘤特异性免疫研究提供了有力的实验材料。Objective To prepare anti-MUC1 core peptide polyclonal antibody used as target in immunotherapy of tumors.Methods Immunize rabbits with recombinant 8R-MUC1 core peptide protein with s ix tandem repeat sequence,and determine the titer of polyclonal antibody against MUC1 by ELISA.The specificity of antibody were identified by Western blot and b locking ELISA separately.Results The polyclonal antibody agains t MUC1,with a titer of 1∶256 000,bound specifically to MUC1 core peptide. Conclusion The 8R-MUCPT protein expressed in prokaryotic system effe ctively induced specific antibody against MUC1 core peptide in immunized animals .It provided an important experimental materials for the study on tumor-targeti ng MUC1 core peptide in immunotherapy. [

关 键 词：MUC1 多克隆抗体 靶向 肿瘤 效价 抗体特异性 特异性抗体 试验鉴定 家兔 诱导免疫 

分 类 号：Q786[生物学—分子生物学] R730[医药卫生—肿瘤]