非水相毛细管电泳-紫外检测法的应用研究——桂枝中肉桂酸的测定  

Study of application of non-aqueous capillary electrophoresis ——ultravoilet detection ——Determining of cinnamic acid in cassia twig

作　　者：李利军[1] 黄文艺[2] 孔红星[1] 程昊[2] 吴健玲[2] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006 [2]广西大学化学与化学工程学院,广西南宁530005

出　　处：《广西工学院学报》2004年第4期1-4,共4页Journal of Guangxi University of Technology

基　　金：广西教育厅科研基金资助项目(2000392).

摘　　要：利用非水体系毛细管电泳-紫外检测法对肉桂酸进行分离与测定,建立了肉桂酸测定的新方法,并研究了非水介质、电解质、运行电压等分离因素对该方法的影响。在25℃下,用未涂层石英毛细管(45cm×75μm,有效长度为30cm),以20mmol/L乙酸铵+30mmol/L乙酸钠的乙醇溶液为电泳缓冲介质,重力进样30s,运行电压为-25KV,检测波长为271nm,测定肉桂酸。以吸收峰的峰面积定量,制作标准工作曲线,其线性范围为4.00～80.00mg/L,相关系数r=0.9997。应用于桂枝中肉桂酸的分离测定,结果较为满意。The new method of determining cinnamic acid was ste up by using the method of non-aqueous system——ultravoilet detection to separate cinnamic acid and detect it.Studied were the effects of separating factors such as non-aqueous medium,electrolyte and notion voltage on the method.Under the temperature of 25℃ the cinnamic acid was determined by using the last coat quartz capillary (45 cm×75 μm,effective length 30 cm),and using the ethanol solution containing 20 mmoI/L ammonium acetate and 30 mmoI/L sodium acetate as electrophoresis buffer medium.And gravitational laying sample is 30 s,motion voltage -25 KV and detecting wave length 271 cm.The standard working curves were made by fixed quantity of the peak area of absorbing peaks,and its linear range is 4.00～80.00 mg/L,the correlation coefficient is r=0.9997.The method is of good results when it is applied to separating and detecting cinnamic acid in the cassia twig.

关 键 词：桂枝 肉桂酸 毛细管电泳 分离 紫外检测 检测波长 测定 非水体系 进样 水相 

分 类 号：O647.3[理学—物理化学] R286.0[理学—化学]