消癃通闭对前列腺平滑肌中NOS神经的影响  

AD experimental study of the effect of XLTB on NOS - containing nerves in prostate smooth musculature cells.

作　　者：贾金铭[1] 马春涛[1] 王勒渝[2] 毕振春[2] 

机构地区：[1]中国中医研究院广安门医院泌尿科,100053 [2]中国中医研究院广安门医院病理科,100053

出　　处：《现代泌尿外科杂志》2001年第2期12-15,共4页Journal of Modern Urology

基　　金：国家自然科学基金

摘　　要：目的 探讨一氧化氮合酶(NOS)与良性前列腺增生(BPH)发病的关系以及中药消癃通闭对前列腺平滑肌中NOS神经的影响。方法 去势雄性大鼠,皮下注射丙酸睾酮5mg·kg^(-1)·d^(-1),分别给予消癃通闭、保列治灌胃,21天后断髓处死,应用NADPH组化染色结合形态学定量分析方法,检测各组前列腺中NOS神经含量。结果 NOS神经纤维主要分布在前列腺平滑肌细胞周围,泪癃通闭高剂量组在治疗3周后,前列腺组织中NOS神经的长度密度(L_v)为0.11349±0.023296,与其它三组相比均有显著性差异(p<0.001)。结论 中药消癃通闭可增加实验性BPH大鼠前列腺中NOS神经含量,为该药治疗前列腺增生,改善临床症状提供了科学依据。Objective To explore the role of nitric oxide synthase(NOS) in the development oi benign prostatic hyperplasia BPH and the effect of Xiao - long- tong- bi(XLTB), a Chinese herb medicine,on NOS-containing nerves in prostatic tissues. Methods The male castrated rats were left intact,given XLTB or finas-teride,and the prostates were excised after 21 days.NADPH diaphorase histochemistry and quantitative,image analysis were used to quantify the NOS - containing nerves in all prostatic tissues. Results The NOS was mainly histochemically localized to nerve fibers coursing through the smooth musculature of the stroma. After 21 days, the length density of NOS - containing nerves in the prostate with XLTB was 0.11349±0.023296, which has significant difference compared with other three groups (p<0.001). Conclusions XLTB increased the NOS- containing nerves in the prostatic tissues of rats, which provides a firm ground for the use of XLTB in the treatment of BPH.

关 键 词：一氧化氮合酶(NOS) 神经纤维 前列腺 平滑肌细胞 BPH 治疗 中药 NADPH 雄性大鼠 丙酸 

分 类 号：R697[医药卫生—泌尿科学] R285.5[医药卫生—外科学]