山东主要绵羊品种线粒体细胞色素b的多态性研究  

STUDIES ON POLYMORPHISMS OF MITOCHONDRIAL CYTOCHROME B GENE SEQUENCES OF MAIN NATIVE SHEEP BREEDS IN SHANDONG

作　　者：张传生[1] 耿立英[2] 杜立新[3] 

机构地区：[1]山东农业大学生命科学学院,山东泰安271018 [2]山东农业大学动物科技学院,山东泰安271018 [3]中国农业科学院畜牧研究所,北京100094

出　　处：《山东农业大学学报（自然科学版）》2004年第4期481-484,共4页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：国家自然科学基金项目 (编号 30 2 70 94 9);山东省自然科学基金项目 (编号 :Y97D0 30 6 2 )资助~~

摘　　要：本文测定了小尾寒羊、大尾寒羊、洼地绵羊 3种动物 4 15bp的线粒体DNA细胞色素b基因片段 ,共发现 7个变异位点 ,变异率为 1.15 %。结果显示 :山东 3个绵羊品种平均差异为 0 .0 115 ,线粒体DNA多态性相对贫乏 ,洼地绵羊与大尾寒羊亲缘关系最近 ,与小尾寒羊的亲缘关系较远 ,推测三个品种具有共同母系祖先。In this study ,fragments of mitochondrial DNA cytochrome b gene (each 415 base pairs in length ) have been characterized from Little-tailed sheep, Large-tailed sheep ,Wadi sheep and by sequencing of the recombinant plasmids ,of which 7 variable sites were identified after combining and aligning the data with the homologous sequence .And the value of average sequence variation in sheep population (π) is 1.15% .Our results suggest that the genetic diversity within the sheep is remarkably scarce and these sheep breeds may have originated from a late common ancestor. Phylogenetic trees were constructed with neighbor joining method with the Lubei White goat as outgroup. The phylogenetic relationships of mitochondrial DNA suggested that Wadi sheep be more closely related with Large-tailed sheep bread.

关 键 词：品种 洼地绵羊 大尾寒羊 小尾寒羊 母系 体细胞 位点 多态性研究 线粒体 异位 

分 类 号：S826[农业科学—畜牧学] S858[农业科学—畜牧兽医]