SMMC7721肝癌细胞不等分次剂量照射后效果的实验研究  

Experimental research on effects of fractional irradiation with variable fraction sizes on SMMC7721 liver malignancy cells

作　　者：缪旭东[1] 鞠永健[1] 张良安[2] 王高仁[1] 吴迪军[1] 赵峰[1] 

机构地区：[1]江苏省南通市第一人民医院放疗科,226001 [2]中国医学科学院中国协和医科大学放射医学研究所

出　　处：《中华放射医学与防护杂志》2004年第6期523-524,共2页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目 (3 9670 2 2 8)

摘　　要：目的　用SMMC772 1肝癌细胞研究不等分次剂量 (VFS)照射方案的效果。方法　用不同的分次剂量方案照射相同细胞数目的不同样本 ,用噻唑蓝 (MTT)分析方法获得照射后的细胞存活率 (S)以进行比较。结果　所有VFS方案照射后获得的S值均小于等分次剂量 (CFS)方案照射后的S值 ,其中部分结果之间的差距有显著的统计学意义 ;在相同变化幅度的情况下 ,递减的VFS照射方案所获得的效果要好于递增VFS照射方案 ,其中 ,部分组别之间的结果有显著的统计学意义。结论实验结果与理论计算结果一致 ,所以 ,VFS方案 ,尤其是分次剂量递减的VFS方案可能应用于临床以提高治疗效果。Objective To analyze the effects of fractional irradiation(FI) with variable fraction sizes(VFS) on SMMC7721 liver malignancy cells. Methods Samples with the same number of SMMC7721 cells were irradiated with different FI protocols,then MTT analysis method was used to determine the cell survival probability after irradiation and comparison was made. Results The S values of the samples irradiated with VFS protocols were all lower than those of the samples irradiated with constant fraction sizes(CFS) protocol,and the differences between part of results were of statistical significance. And for the same variety range,the effect of FI with decreasing fraction sizes was better than that of FI with increasing ones,also the differences of S values were of statistical significance. Conclusion The result of our experiment using SMMC7721 cells is coincident with the former calculation results,indicating the VFS irradiation protocols,especially the protocol with decreasing fraction slzes,can be used in the clinic to improve the curative effect.;

关 键 词：照射 剂量 SMMC7721 肝癌细胞 治疗效果 实验研究 噻唑蓝 后效 细胞数目 细胞存活率 

分 类 号：R735.7[医药卫生—肿瘤] R730[医药卫生—临床医学]