电离辐射对小鼠组织金属硫蛋白-1mRNA表达的影响  

Effect of ionizing radiation on MT-1 mRNA expression of mouse tissues

作　　者：范才[1] 张建军[1] 李秀丽[1] 徐莉[1] 陈卫辉[1] 鞠桂芝[2] 

机构地区：[1]白求恩国际和平医院放疗科,石家庄050082 [2]吉林大学放射生物学教研室

出　　处：《中华放射医学与防护杂志》2004年第6期525-526,共2页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目 (3 9170 2 75 )

摘　　要：目的　探讨X射线全身照射对小鼠肝、脑、胸腺及脾脏金属硫蛋白 (MT) 1mRNA表达的影响及其剂量效应关系。方法　采用Northern杂交方法检测了X射线全身照射后小鼠肝、脑、胸腺及脾脏MT 1mRNA水平变化。结果　 4GyX射线照射后肝脏及胸腺MT 1mRNA水平逐渐升高 ,照射后 4h达峰值 ,分别为假射组的 1 82及 1 72倍 ,2 4h基本恢复至正常水平 ;0 5～ 6Gy照射后 4h ,肝脏及胸腺MT 1mRNA水平均呈剂量依赖性增加 ,6Gy照射后MT 1mRNA水平分别为假照组的2 13和 1 6 2倍。但X射线全身照射后小鼠脑及脾脏MT 1mRNA水平未见明显变化。结论　X射线全身照射可提高小鼠肝脏和胸腺MT 1mRNA水平 ,但对小鼠脑及脾脏MT 1mRNA表达无影响。Objective To investigate the effect of whole body X-irradiation (WBI) on metallothionein-1 (MT-1) gene transcription level in mouse tissues and its dose-effect relationship. Methods MT-1 mRNA levels in mouse liver,brain,thymus and spleen were measured using Northern blot assay. Results Time-course experiment showed that MT-1 mRNA levels in mouse liver and thymus gradually increased after WBI,reached a peak at 4 h after WBI and recovered normal level at 24 h. MT-1 mRNA levels in mouse liver and thymus increased with increase of irradiation dose at 4 h after 0.5～6 Gy X-ray WBI. But there was no obvious change of MT-1 mRNA levels in mouse brain and spleen. Conclusion MT-1 mRNA levels in mouse liver and thymus significantly increase after WBI and showed a dose-dependent trend. But there is no obvious change in MT-1 mRNA levels in mouse brain and spleen. These results indicate that increase of MT-1 mRNA in mouse tissues after WBI is tissue-specific,and MT protein synthesis is regulated through MT-1 gene transcriptional elevation.;

关 键 词：小鼠 MT 胸腺 MRNA表达 脾脏 鼠肝 MRNA水平 金属硫蛋白 电离辐射 照射 

分 类 号：R361[医药卫生—病理学]