钻天松无菌系建立的初步研究  

Priliminary Study on Construction of Pinus sylvestris var.Fastigiata

作　　者：徐连峰[1] 李长松[2] 赵凌泉[1] 史绍林[1] 应亚琳[1] 

机构地区：[1]黑龙江省森林与环境科学研究院,黑龙江齐齐哈尔161041 [2]东北林业大学

出　　处：《防护林科技》2005年第2期12-13,共2页Protection Forest Science and Technology

摘　　要：摘　要:以钻天松侧枝顶芽作为繁殖材料,通过不同消毒剂处理试验,不同生长激素对外殖体愈伤组织形成的诱导,初步建立无菌系。结果表明:0.1%HgCl2 消毒时间最好为9 min;时间短,污染率高;时间过长会导致芽中毒而成活率下降。诱导钻天松愈伤组织形成的最佳培养基配方是1/2MS+ 6BA0. 2mg·L-1 + NAA0. 5mg·L-1 +IBA0.2mg·L-1,低浓度的6BA有利于愈伤组织的形成。Terminal bud of lateral branch of Pinus sylvestris var.fastigiata was used as propagation Materials,callus induction of explant treated by growth hormone was studied.Aseptic strain of Pinus sylvestris var.fastigiata was constructed.The results showed that the optimum sterilizing time of terminal bud with the concentration of 0.1%HgCl 2 would be 9 min.Short time could cause high pollution and long time could cause toxic bud and lower survival ratio.The optimum recipe of culture medium culture which was induced by callus culture of Pinus sylvestris var.fastigiata was 1/2MS+6BA0.2mg·L^(-1)+ NAA 0.5mg·L^(-1)+IBA0.2 mg·L^(-1).Lower concentration of 6BA would be favourable to form callus.

关 键 词：无菌系 愈伤组织 侧枝 成活率 消毒时间 外殖体 培养基配方 初步研究 污染率 生长激素 

分 类 号：S661.2[农业科学—果树学] S681.4[农业科学—园艺学]