核糖核酸酶抑制因子在肺癌中的表达  

The Expression of Ribonuclease Inhibitor in Lung Cancer

作　　者：许凝[1] 孙兵[2] 李继良[2] 

机构地区：[1]大连大学附属医院心胸外科,辽宁大连116021 [2]大连医科大学附属一院心胸外科

出　　处：《中国误诊学杂志》2005年第3期426-428,共3页Chinese Journal of Misdiagnostics

摘　　要：目的通过核糖核酸酶抑制因子(ribonucleaseinhibitor,RI)基因在人肺癌及同体癌旁正常肺组织中的表达的检测,进一步探讨RI与恶性肿瘤细胞生长的关系。方法应用Westernblotting方法检测人肺癌组织中RI的蛋白表达量;应用RT-PCR的方法检测人肺癌组织中RI的mRNA的表达量。采用χ2检验(chi-squaretest)分析获得的数据,用SPSS10.0统计软件进行处理。结果人肺癌组织中RI的蛋白及mRNA的表达均明显低于同体癌旁正常肺组织。结论核糖核酸酶抑制因子(RI)在肺癌中的表达下调,肺癌的生长可能与RI表达的下调有关。Objective:To explore the relationships between RI and the growth of tumor cells by determining the expressionof RI gene in carcinoma and in normal tissue.Methods:Western blotting was used to examine the protein expression of R I and the result was shown by photograth. RT-PCR was used to detect the mRNA ex pression of RI and the result was examined by electrophoresis. Chi-square test was used to analyze the experiments data.Results:The expressions of RI protein and mRNA in lung cancer tissues were significantly lower than in normal lung tissues.Conclusion:The expression of RI was declined in lung cancer.The growth of lung cancer should be probably associated with the decline expression of RI.

关 键 词：核糖核酸酶抑制因子 肺肿瘤/代谢 基因表达 

分 类 号：R734.2[医药卫生—肿瘤]