有机溶剂对苏云金芽孢杆菌(Bacillus thuringiensis)几丁质酶的影响  

EFFECTS OF DIFFERENT ORGANIC SOLVENTS ON THE CHITINASE FROM BACILLUS THURINGENESIS

作　　者：黄小红[1] 陈清西[2] 王君[2] 沙莉[1] 黄志鹏[1] 关雄[1] 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室 [2]厦门大学生命科学学院教育部细胞生物学与肿瘤细胞工程重点实验室,福建厦门361005

出　　处：《应用与环境生物学报》2005年第1期71-73,共3页Chinese Journal of Applied and Environmental Biology

基　　金：国家高技术研究发展计划 (2002AA245011 );福建省重大科技计划项目(2002N004)资助 ~~

摘　　要：　以苏云金芽孢杆菌(Bt)的发酵液为材料,经硫酸铵分级分离、DEAE-32离子交换柱层析分离,获得部分纯化的Bt几丁质酶(EC3. 2. 1. 14)制剂.研究了几种有机溶剂对Bt几丁质酶的影响.结果表明,甲醇、丙三醇、甲醛和戊二醛对几丁质酶有抑制作用;乙醇、丙醇、乙二醇和二甲亚砜在低浓度时对酶有激活作用,随着浓度的升高表现出抑制作用;二氧六环的浓度低于 5%时,对酶的影响不明显,而高于 5%时,对酶则有激活作用;丙酮对酶有激活作用. 图 1参A chitinase (EC3.2.1.14) was isolated from the culture of Bacillus thuringenesis and purified by ammonium sulfate fractionation and chromatography on DEAE-32. The effects of organic solvents on the enzyme activity were investigated. The results indicated that the methanol, glycerol, formaldehyde and glutaraldehyde solutions inhibited the enzyme activity, while the alcohol, propanol, glycol and dimethyl sulfoxide solutions activated the enzyme at low concentration, and inhibited it at high concentration. Acetone and dioxane had active effects on the enzyme. Fig 1, Ref 10

关 键 词：苏云金芽孢杆菌 几丁质酶 有机溶剂 酶活力 

分 类 号：S482.39[农业科学—农药学]