Northern杂交检测多肽:N-乙酰氨基半乳糖转移酶2在不同肿瘤细胞中的表达水平  

Detection on Expressive level of pp-GalNAc-T2 in Different Tumor Cells by Northern-blotting

作　　者：仇灏[1] 陈克平[1] 周嘉梁[1] 彭淼[1] 吴士良[1] 

机构地区：[1]苏州大学基础医学系生化与分子生物学教研室生化工程研究所,江苏苏州215007

出　　处：《江苏大学学报（医学版）》2005年第1期14-16,共3页Journal of Jiangsu University：Medicine Edition

基　　金：国防基础科研计划资助项目(K0102061501)苏州大学医学发展基金资助项目(2003007)。

摘　　要：目的:从mRNA水平检测五种不同肿瘤细胞株中多肽:N-乙酰氨基半乳糖转移酶2(pp-GalNAc-T2)的表达情况。方法:采用Northern杂交方法。结果:Northern杂交结果显示了两条区带,并且pp-GalNAc-T2在不同肿瘤细胞中存在表达差异。结论:pp-GalNAc-T2基因在不同细胞存在差异表达并可能存在着剪切现象。Objective: To detect the mRNA expression of polypeptide; N-acetylgalactosaminyltransferase2 (pp-GalNAc-T2) in different tumor cells. Methods: Using the method of Northern-blotting. Results: Northern hybridization showed two bands in each cell, and there was expressive discrepancy of pp-GalNAc-T2 in different tumor cells. Conclusion: There was expressive discrepancy of pp-GalNAc-T2 in different tumor cells. And the splicing of mRNA of pp-GalNAc-T2 occured in several cells.

关 键 词：半乳糖 转移酶 肿瘤细胞株 NORTHERN杂交 多肽 表达水平 MRNA水平 表达差异 差异表达 基因 

分 类 号：R735[医药卫生—肿瘤] R730[医药卫生—临床医学]