Pilot Study of Molecular Mechanism on Vasculogenic Mimicry in Bi-directional Differentiated Malignant Tumors  

双向分化肿瘤血管生成拟态分子机制初步观察(英文)

作　　者：孙保存[1] 张诗武[1] 赵秀兰[1] 张丹芳[1] 郝希山[1] 

机构地区：[1]天津市肿瘤医院肿瘤研究所病理科,300060

出　　处：《The Chinese-German Journal of Clinical Oncology》2005年第1期50-52,68,共4页中德临床肿瘤学杂志（英文版）

基　　金：This work was partially supported by a grant from the National Natural Science Foundation of China (No. 30370378)

摘　　要：Objective: To investigate the role of collagen IV and PAS positive substancesecreted by tumor cells in vasculogenic mimicry (VM) and the effects of VM on tumor cells expressingVEGF. Methods: 158 cases of bi-direction differential malignant tumor specimens withparaffin-embedded were enrolled into our study and made tissue microarray which were dual-stainedwith CD31-PAS and stained with collagen IV. The difference of the areas and distribution withpattern surrounded by between CD31 and PAS positive respectively were identified via grid-counting,as well as the difference of VEGF expression with VE absent and present. Results: The basementmembrane of VM was both PAS and collagen IV positive. VEGF expression in the bi-directiondifferential malignant tumor was higher VM-absent than VM-present and the difference wasstatistically significance in malignant melanoma and alveolar rhabdomyosarcoma (P 【 0.05).Conclusion: PAS positive substance and collagen IV compose the wall of VE and VE could provide theoxygen and nutrition for tumor growth and progression.

关 键 词：bi-direction differential malignant tumor vasculogenic mimicry dual-staining tissue microarray 

分 类 号：R730.2[医药卫生—肿瘤]