非4℃条件延长红细胞保存有效期的探讨  

Prolonging the Shelf Life of RBCs Stored at Non-4℃

作　　者：刘景汉[1] 韩玮[1] 于洋[1] 庄远[1] 

机构地区：[1]解放军总医院输血科,解放军临床输血中心,北京100853

出　　处：《中国医师杂志》2005年第2期166-168,共3页Journal of Chinese Physician

基　　金："十五"军队医药卫生科研基金指令性课题 (0 1L0 4 5)

摘　　要：目的　探讨有效延长非 4℃条件下红细胞保存有效期 ,提高战时血液保障能力。方法　采集 12名健康捐血者的血液各 40 0ml并制备浓缩红细胞 ,每袋平均分成 5袋 ,标记为联合组、新配方组、氨组、对照组和 4℃组 ,各组分别添加新保存液及氨、新保存液、氨、SAGM保存液、SAGM保存液 ,前四组置室温保存 ,每天取样 ,4℃组放入 4℃冰箱保存 ,在保存末期即 3 5d取样 ,分别测定红细胞ATP等保存指标。以 4℃组ATP为临界ATP ,观察各组达到临界ATP值的保存时间 ,确定各组保存有效期。取 6袋浓缩红细胞 ,按照联合组标准添加保存液 ,室温保存 12d后每袋平均分成 4袋 (组 )并分别洗涤 ,离心前分别平衡 0min、5min、10min和 15min ,监测残留NH4+ 和红细胞回收率。结果　联合组、配方组、NH4+ 组和SAGM组的保存有效期分别为 12、6、6和 5d ;若平衡 5min ,红细胞洗涤 4次可有效去除残留NH4+ ,平衡时间达到 10min或 15min洗涤 3次即可达到目的 ,洗涤 3次和 4次红细胞回收率分别达到88 5 %和 83 1%。结论　联合配方是常温下较为理想的红细胞保存液 ,使用生理盐水洗涤可有效地清除血氨。Objective To prolong the shelf life of RBCs stored at non-4℃, and improve the ability of blood supplying during wartime. Methods 12 bags of packed RBC were collected from 12 volunteers presenting blood, respectively, and each was averagely divided into 5 parts(groups): combination group, new solution group, ammonia group, control group and 4℃ group. The fist 4 groups were stored at room temperature, and were tested for ATP concentration daily. 4℃ group was stored at 4℃, and RBC ATP concentration was measured at the end of its shelf life, which served as a critical ATP concentration. When RBC ATP concentration of the fist 4 groups decreased to the level of critical ATP, the time of preservation was their shelf life. 6 bags of packed RBC were added the same solution as the combination group, and each was averagely divided into 4 parts(groups)after stored at room temperature for 12 days, which were washed for 0, 5,10 and 15min before centrifugation, respectively. The residual ammonia and the recovery ratio of RBC were measured after washing. Results The shelf life of combination group,new solution group,ammonia group and control group was 12,6,6 and 5 days, respectively. The residual ammonia could be effectively cleaned up after either washing 4 times with 5 min balance time or 3 times with 10 or 15 min balance time. The recovery ratio of RBC was 88.5% and 83.1% after washing 3 and 4 times, respectively. Conclusion The combination of new solution and ammonia was a good additive solution for RBC preservation at non-4℃, and the residual ammonia can be cleaned up using normal saline.

关 键 词：浓缩红细胞 联合 有效期 保存液 室温保存 血液 延长 平均分 条件 结论 

分 类 号：R457[医药卫生—治疗学] R446[医药卫生—临床医学]