中华绒螯蟹不同发育时期胚胎及流产胚胎的同工酶变化  

Isozyme Changes in Different Stage Embryos and Aborted Embryos in Eriocheir sinensis

作　　者：贾守菊[1] 应雪萍[1] 陈艳乐[1] 应建萍[1] 

机构地区：[1]温州师范学院生命与环境科学学院,温州325027

出　　处：《动物学杂志》2005年第1期76-83,共8页Chinese Journal of Zoology

基　　金：温州市科技局基金资助项目 (No.N2 0 0 0A2 6 ) ;乐清市科技局基金资助项目

摘　　要：采用聚丙烯酰胺凝胶电泳技术 ,对中华绒螯蟹 (Eriocheirsinensis)不同发育时期胚胎及流产胚胎的6种同工酶 (乳酸脱氢酶、醇脱氢酶、苹果酸脱氢酶、酯酶、淀粉酶和过氧化物酶 )进行了研究。结果表明 ,不同发育时期胚胎的乳酸脱氢酶、醇脱氢酶、苹果酸脱氢酶、酯酶及淀粉酶酶谱表现出一定差异。受精卵中未检测到乳酸脱氢酶同工酶活性 ,卵裂期和囊胚期出现 4条酶带 ,无节幼体及氵蚤状幼体期只有 2条酶带 ;醇脱氢酶同工酶在中华绒螯蟹胚胎发育的各阶段均有表达 ,但表达的酶带数和活性有区别 ;在受精卵和氵蚤状幼体期无苹果酸脱氢酶酶带显示 ,卵裂期酶带数最多 ,酶活性相对也最强 ,以后随着发育的进行 ,酶带数和酶活性都有减弱的现象 ;酯酶同工酶的变化较为复杂 ,特别是囊胚期 ,酯酶酶带突然全部消失 ;淀粉酶有两种类型 :α 淀粉酶和R 淀粉酶。从受精卵发育到幼体其酶带数不增反减。不同发育阶段均检测不到过氧化物酶的活性。第二次抱卵胚胎的酶活性和酶带数低于或有别于第一次抱卵的卵裂期胚胎。流产胚胎中醇脱氢酶、乳酸脱氢酶、淀粉酶与第一次抱卵胚胎不同发育时期的酶谱相比略有变化 ,而苹果酸脱氢酶、酯酶酶带数迅速减少。Six isozymes, LDH, ADH, MDH, EST, AMY, and POD, in different stage embryos and aborted embryos in Eriocheir sinensis were investigated with polyacrylamide gel electrophoresis. The results showed that the activities of LDH, ADH, MDH, EST and AMY were different in embryos at different developmental stages. LDH isozyme could not be detected in fertilized eggs, while four isozyme bands emerged in cleavage and blastulation stage embryos and two isozyme bands in nauplius and zoaea stages; ADH isozyme was expressed in all stages of embryonic development, but there were differences in enzyme bands and enzyme activities. No MDH enzyme bands were observed in fertilized eggs and at zoaea stages, while most bands and the strongest activities emerged at cleavage stages. With the development, the MDH enzyme bands and activities became weakened. The changes in EST isozyme were more complicated, with sudden disappearance at blastulation stage. The band number of AMY, both (α-AMY and R-AMY) decreased from fertilized eggs to larver stage.The POD activity could not be detected in all developmental stages. The enzyme activities and bands of the second spawning embryos were different from those of the first cleavage stageembryos. The ADH, LDH, and AMY isozyme bands of aborted embryos had slight differences compared to the first spawning embryos at different developmental stages, while MDH, EST isozyme bands decreased dramatically in aborted embryos.

关 键 词：发育时期 中华绒螯蟹 酯酶 酶活性 同工酶 酶变化 幼体 胚胎 乳酸脱氢酶 醇脱氢酶 

分 类 号：Q593[生物学—生物化学]