多药耐药相关蛋白基因、肺耐药蛋白基因在肝细胞癌中的表达及临床意义  

Expressions and clinical significance of multidrug resistance associated protein gene (MRP) and lung resistance protein gene (LRP) in hepatocellular carcinoma (HCC)

作　　者：王百林[1] 杨海燕[1] 翟淑萍[1] 陈孝平[2] 仲永[3] 张庆业[1] 孟磊[1] 

机构地区：[1]广州中医药大学第一附属医院三外科,广东广州510405 [2]华中科技大学同济医学院附属同济医院肝脏外科中心,湖北武汉430030 [3]昆明医学院第二附属医院普通外科,云南昆明650000

出　　处：《中国普通外科杂志》2005年第2期95-99,共5页China Journal of General Surgery

摘　　要：　　目的　研究多药耐药相关蛋白基因 (MRP)和肺耐药蛋白基因 (LRP)在肝细胞癌 (HCC)中的表达及其与临床的关系。方法　采用SP免疫组化和PCR技术检测 5 4例HCC和 2 4例癌旁组织及 1 2 例肝炎后肝硬化组织中的两种基因表达。结果　MRP和LRP在 3种组织中均有表达,HCC组织中其基因表达显著高于其他组织 (P< 0. 0 5 )。HCC中两种基因表达呈正相关 (r= 0. 3 1 2, P <0. 0 5; r= 0. 3 2 8, P< 0. 0 5 );其表达与HCC的分化程度有关 (P< 0. 0 5 )。并且其表达阳性组患者化疗后AFP有效率和平均生存期分别高于和长于阴性组 (P< 0. 0 5 ),但LRP与平均生存期无关 (P> 0. 0 5 )。结论　HCC多药耐药 (MDR)与两种基因表达有关,起源于内源性耐药;检测其表达对预测化疗耐药具有指导意义;MRP可能与预后有关。Objective To study the expressions and significance of MRP and LRP in HCC. Methods The expressions of two genes were examined in three tissues (54 cases of HCC, 24 para cancer and 12 posthepatitis cirrhosis tissues) by SP immunohistochemical and PCR techniques. Results MRP and LRP were expressed in three tissues, with significantly higher rates in HCC than others (P<0.05). The MRP expression positively correlated with the LRP expression in HCC (r=0.312, P<0.05; r=0.328, P<0.05), and was related to the degree of HCC differentiation (P<0.05). In the patients with positive gene expression,the AFP decresing rate was higher and the average survival after chemotherapy was longer than the negative expression group(P<0.05),but there was no relationship between LRP expressions and the average survival time . Conclusions Multidrug resistance (MDR) of HCC is related to MRP,LRP expressions , and is initiated by intrinsic MDR. Monitoring of MRP and LRP may help to prospectively evaluate drug resistance. MRP may be relates to the prognosis of HCC patients.

关 键 词：肝肿瘤/病理学 肝细胞瘤/病理学 基因表达 多药耐药相关蛋白基因 肺耐药蛋白基因 

分 类 号：R735.7[医药卫生—肿瘤] R730.261[医药卫生—临床医学]