β-连环素在早期大肠癌中的表达及其意义  

Expression of B-catenin in early colorectal carcinoma and its significance

作　　者：赵勇[1] 章必成[1] 高建飞[1] 李欣[1] 杜光祖[1] 

机构地区：[1]广州军区武汉总医院肿瘤科,武汉市430070

出　　处：《实用医学杂志》2005年第5期458-461,共4页The Journal of Practical Medicine

摘　　要：目的:探讨β-连环素在早期大肠癌发生、浸润和转移中的作用。方法:用免疫组织化学及原位杂交方法检测早期大肠癌及其相应正常肠黏膜、腺瘤、进展期大肠癌β-连环素蛋白及mRNA表达水平。结果:(1)早期大肠癌出现细胞膜表达减少、细胞浆和(或)细胞核阳性着色等异常表达,且异常表达细胞多为癌灶周围的癌细胞。(2)由正常肠黏膜、早期大肠癌、腺瘤至进展期大肠癌β-连环素在细胞膜表达呈下降趋势(P<0.05);在胞浆或胞核表达呈上升趋势(P<0.05)。(3)早期大肠癌中β-cat表达与肿瘤的侵袭深度和转移相关(P<0.05);随着早期大肠癌细胞分化程度的降低,β-连环素在细胞膜表达逐渐减少(P<0.05),而与细胞浆或核表达不相关(P>0.05)。(4)在连续切片中β-连环素mRNA着色部位基本与免疫组化染色相对应。从正常肠黏膜、腺瘤、早期大肠癌到进展期大肠癌β-连环素mRNA表达呈上升趋势(P<0.05)。早期大肠癌中β-连环素mRNA表达与肿瘤侵袭深度和转移相关(P<0.05)。结论:β-连环素可能参与早期大肠癌的发生、浸润和转移。Objective To investigate the correlation of β catenin expression with the progression of early colorectal carcinoma. Methods High sensitive S Pimmunohistochemical method and in situ hybridization were used to detected the proteins and the mRNA of β catenin expression in clinical specimens from normal muscosas , adenomas, early colorectal cancers to advanced colorectal cancers. Results (1)Abnormal β catenin expression with loss of membranous immunoreactivity or cytoplasmic or nuclear staining was observed in some cases of early colorectal cancers. Tumor cells with abnormal expression of β catenin were distributed heterogeneously in the tumor nest and were often located at the invasive front. (2)The reduced membranous expression , the abnormal cytoplasmic or nuclear staining of β catenin was gradually increased from normal muscosas , adenomas, early colorectal cancers to advanced colorectal cancers (P< 0.05). (3) The membranous expression rates of β catenin gradually decreased with tumor cells dedifferentiation(P< 0.05). Cytoplasmic or nuclear expression of β catenin was not significantly associated with histological differentiation (P >0.05). The reduced membranous or cytoplasmic or nuclear expression of β catenin in early colorectal cancer was significantly associated with the depth of invasion and metastasis (P< 0.05). (4) In the consecutive tissue slices , the location of expression of β catenin protein and its mRNA corresponded. The level of β catenin mRNA gradually increased from normal muscosas, adenomas , early colorectal cancers to advanced colorectal cancers (P< 0.05). It was significantly associated with the depth of invasion and metastasis (P< 0.05). Conclusion β catenin may play an important role in the carcinogenesis, infiltration and metastasis of early colorectal cancer.

关 键 词：早期大肠癌 Β-连环素 mRNA表达 胞浆 正常 肠黏膜 腺瘤 细胞膜 细胞核 蛋白 

分 类 号：R735.34[医药卫生—肿瘤] R737[医药卫生—临床医学]