原发性乳腺癌10q23区域等位基因缺失对FAK磷酸化影响  

Allelic loss of the 10q23 region in primary breast cancer

作　　者：朱宁生[1] 江歌丽[1] 杨稼祥[1] 刘晓俞[1] 肖觉[1] 张代奎[1] 

机构地区：[1]重庆市肿瘤医院乳腺科,400030

出　　处：《重庆医学》2005年第3期409-411,共3页Chongqing medicine

摘　　要：目的　了解10q23区域的杂合型缺失(loss of heterozygosity,LOH)对原发性乳腺癌FAK磷酸化的影响。方法　采用位于10q23的4对微卫星标记物,通过PCR方法检测杂合型缺失;免疫组化方法检测PTEN和Western blot检测FAK蛋白质表达和磷酸化。结果　44例样本中,14例至少发生一个微卫星标记物的杂合型缺失,LOH发生率为 31.82%(14/44)。在 14 例发生LOH的病例中,57.14% (8/14)PTEN蛋白质表达缺失, 64.29%(9/14)发生 FAK磷酸化。结论　10q23 区域等位基因的缺失降低了PTEN的表达,进而影响FAK磷酸化程度。Objective To investigate the relation between LOH (loss of heterozygosity) in loci of 10q23 region and FAK phosphorylation in primary breast cancer.Methods LOH analysis of the 10q23 region was performed by PCR, using four microsatellite markers of the 10q23 region. The protein expression of PTEN was detected by immunohistochemistry.FAK phosphorylation was investigated by Western blot.Results LOH in at least one marker of the 10q23 region was found in 31.82% of patients(14/44). Loss or dramatically low expression of PTEN protein was detected in 57.14% (8/14) of patients. FAK phosphorylation occurred in 64.29% (9/14) of patients. Patients with LOH in the loci of 10q23 were significantly associated with loss of the expression of PTEN protein and FAK phosphorylation (P<0.05).Conclusion The LOH in 10q 23 region, which affected the protein expression of PTEN, might take its effects on FAK phosphorylation that involved in the metastasis of primary breast cancer.

关 键 词：原发性乳腺癌 LOH PTEN FAK 

分 类 号：R737.9[医药卫生—肿瘤]