子房注射法将Bt基因导入超甜玉米  

Transformation of Bt Gene into Elite Supersweet Maize Hybrid by Ovary Injection

作　　者：李余良[1] 胡建广[1] 苏菁[2] 刘建华[1] 

机构地区：[1]广东省农科院作物研究所,广州510640 [2]中山大学生命科学学院,广州510275

出　　处：《玉米科学》2005年第1期41-43,共3页Journal of Maize Sciences

基　　金：广东省"十五"重大科技专项(A202004);广东省农科院重点科技攻关项目(2001攻关-08);广东省农科院院长基金(2004-02)资助

摘　　要：用子房注射法将Bt基因导入超甜玉米品种粤甜3号,获得了1株整合有Bt基因的转基因植株,定名为ZT11,以发芽成苗的植株计算,基因转化效率达9.09%。经PCR和Southernblot分析,证明抗虫基因已经整合在超甜玉米基因组中,并且为单拷贝。T1代植株PCR检测结果表明,Bt基因能够在转基因后代中稳定遗传,基因分离符合1∶1的孟德尔遗传分离规律。Bt gene was successfully transferred into elite supersweet maize hybrid by overy injection in the field, and one transgenic plant ZT11 integrated with Bt gene was obtained. By PCR amplification and southern blot analysis, the extrogenous gene with specific promoter was confirmed to integrate into the supersweet maize genome DNA with single-copy, and transformation frequency could reach as high as 9.09%. The molecular detection of T1 progeny proved that mostly transgenic plant could inherit steadily and their segregation pattern fell into Mendel mode 1∶1.

关 键 词：超甜玉米 BT基因导入 植株 子房注射法 成苗 转基因后代 抗虫基因 孟德尔 ZT 基因组 

分 类 号：S513[农业科学—作物学] S562