神经生长因子对大鼠心肌梗塞后胆碱能神经的保护作用  

Effect of Nerve Growth Factor on Cardiac Cholinergic Innervation in Rat with Myocardial Infarction

作　　者：倪秀芹[1] 李星 薛红杰[1] 赵玲辉[1] 刘式威[3] 马晶[1] 徐嘉惠[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,黑龙江哈尔滨150086 [2]鸡西电力医院 [3]解放军211医院

出　　处：《解剖科学进展》2005年第1期22-24,共3页Progress of Anatomical Sciences

基　　金：黑龙江省科技厅攻关课题(GOOC190906)

摘　　要：目的　观察神经生长因子(NGF)对大鼠心肌梗塞后胆碱能神经的影响,探讨NGF对心肌梗塞的可 能治疗作用。方法　SD大鼠20只制心梗模型,其中10只心梗后给予NGF为实验组,2天后取材,同时取正常心肌 作对照。以Karnovsky Roots法,显示胆碱能神经纤维,应用多功能真彩色病理图像分析系统分析胆碱能神经纤维 密度。结果　心梗左心室心肌梗塞区及梗塞周围区存活心肌中胆碱能神经纤维密度比正常组明显降低(P<0. 01);实验组应用NGF后胆碱能神经纤维密度较心梗组升高。结论　神经生长因子能增加大鼠心肌梗塞后胆碱能 神经纤维的密度,可能对心梗有治疗作用。Objective To observe the effect of nerve growth factor (NGF) on cardiac cholinergic innervation in rat with myocardial infarction (AMI) to oxplore the effect of NGF in treatment of AMI. Methods AMI was made in 20 SD rats, NGF was injected intravenously into 10 of the 20 AMI rats. Karnovsky-Roots method was used to show the distribution of cholinergic fibers and the density of the fibers was analyzed by medical pathological image analysis processing system. Results The density of the cholinergic fibers in AMI rats is less than that in normal rats. The density of the cholinergic fibers in experimental group using NGF is much higher than that in AMI group. Conclusion NGF increases the density of the cholinergic fibers in AMI rat and might have the possible effect on the treatment of AMI.

关 键 词：胆碱能神经 心梗 心肌梗塞 NGF 神经生长因子 大鼠 正常 目的观 结论 取材 

分 类 号：Q421[生物学—神经生物学] R542[生物学—生理学]