大鼠脑缺血再灌流后室旁核大细胞的超微结构变化  

Ultrastructural Changes of the Paraventricular Nucleus (PVN) of Rat after Global Ischemia-reperfusion

作　　者：吴江锋[1] 张赫焱[2] 张艳琼[3] 吴超[3] 姚志芳[3] 

机构地区：[1]三峡大学医学院解剖教研室,湖北宜昌443003 [2]吉林大学中日联谊医院中医科 [3]三峡大学医学院

出　　处：《解剖科学进展》2005年第1期40-42,共3页Progress of Anatomical Sciences

基　　金：三峡大学青年科学基金资助(KJA0203);三峡大学重大科学基金资助(KJA0301)

摘　　要：目的　观察脑缺血再灌流后室旁核的大细胞的超微结构变化。方法　复制大鼠全脑缺血再灌 动物模型,超薄切片,醋酸双氧铀和柠檬酸铅双重染色,JEM 1200透射电镜观察。结果　与正常大鼠比较, 鼠全脑缺血再灌注后室旁核大细胞的核染色质和核膜变化明显,细胞器变化的先后顺序是滑面内质网扩张 线粒体嵴不清或消失,细胞质空泡化,后出现部分细胞器的解体。结论　脑缺血再灌流引起室旁核大分泌 经元出现超微结构的动态变化。Objective To observe the ultrastructural changes of the magnocellars of PVN in Wistar rat after global ischemia-reperfusion.Methods The ultrathin section, uranyl acetate and citromalic acid lead double stainings and JEM-1200 electron microscope were used.Results Compared with normal rats, the changes of the nuclear chromatin and nuclear membrane of the magnocellar cells of PVN in ischemia reperfusion rats were obvious. The order of the changes of organelles was as follows: the expanded endoplasmic reticulum, the swollen mitochondria and unclear or disappearance of the mitochodria crest, and finally the dissolvement of the organelles and the cells in magnocelular cells of PVN in ischemia reperfusion rats.Conclusion Ischemia reperfusion damaged the magnocellular cells of PVN with the more serious degrees as the time lasted from the third day to the sixth day after ischemia reperfusion.

关 键 词：室旁核 大细胞 脑缺血再灌流 正常大鼠 全脑缺血再灌注 动物模型 双重染色 细胞器 核膜 染色质 

分 类 号：Q959.483[生物学—动物学] R743[医药卫生—神经病学与精神病学]