碱性磷酸酶同工酶对原发性胆汁性肝硬化的诊断价值初探  

Investigation on the diagnostic value of alkaline phosphatase isoenzymes in the primary biliary cirrhosis

作　　者：丁媛媛[1] 杨蔺[1] 郑慧雅[1] 张永为[2] 陈福祥[1] 

机构地区：[1]上海市东方医院检验科,上海200120 [2]江苏大学医学技术学院,实习生江苏镇江212001

出　　处：《检验医学》2005年第2期140-142,共3页Laboratory Medicine

摘　　要：目的　初步探讨血清碱性磷酸酶(ALP)同工酶对原发性胆汁性肝硬化(PBC)的辅助诊断价值。方法 　用Sebia琼脂糖凝胶电泳分离30名正常人、23例PBC患者和25例其他肝胆疾病患者的血清ALP同工酶,经 光密度扫描仪扫描后,分析各型同工酶的相对含量。结果　血清ALP同工酶经电泳后可分为肝型、胆汁型、肠 型和骨型4个区带。PBC患者的肝型ALP的相对含量最高,约为56%～70%,显著高于正常对照及乙肝后肝硬 化患者(P<0.05),而其胆汁型ALP的相对含量显著低于肝外阻塞性黄疸患者(P<0.05)。结论　ALP同工酶 分析可辅助诊断PBC。Objective To investigate the value of alkaline phosphatease (ALP) isoenzymes determination in the diagnosis of primary biliary cirrhosis(PBC). Methods ALP isoenzymes were measured by agarose gel electrophoresis in the sera from 30 control subjects, 23 patients with PBC and 25 patients with other hepatobiliary disease. Results ALP isoenzymes could be divided into 4 separate sections: liver type, bile type, intestine type and bone type. The mean level of liver type ALP isoenzyme in patients with PBC was significantly higher than that of control groups and hepatocirrhosis patients (P<0.05), but the mean level of bile type ALP isoenzyme in patients with PBC was lower than that of extrahepatic obstruction(P<0.05). Conclusions ALP isoenzymes determination can be useful in the diagnosis of PBC.

关 键 词：碱性磷酸酶 同工酶 原发性胆汁性肝硬化 诊断 Sebia琼脂糖凝胶电泳 

分 类 号：R575.2[医药卫生—消化系统]