Preparation and evaluation of As_2O _3 nanoparticles for treatment of human liver cancer cells in vitro  

As_2O_3纳米粒的制备、表征和体外治疗人肝癌细胞的研究(英文)

作　　者：王子妤[1] 张东生[1] 顾宁[2] 路新丽[1] 颜士岩[1] 

机构地区：[1]东南大学基础医学院,南京210009 [2]东南大学生物科学与医学工程系,南京210096

出　　处：《Journal of Southeast University(English Edition)》2005年第1期58-62,共5页东南大学学报（英文版）

基　　金：TheNationalNaturalScienceFoundationofChina(No.30371830),theNationalHighTechnologyResearchandDevelopmentProgramofChina(863Program)(No.2002AA302207),theNaturalScienceFoundationofJiangsuProvince(No.BK2001003),theKeyProjectofChineseTraditionalMedicineofJiangsuProvince(No.H027).

摘　　要：This paper studies the preparation method of As_2O_3 nanoparticles and theirantitumor effect on human liver cancer cells. As_2O_3 nanoparticles were prepared by sol-gel method.As_2O_3 nanoparticles were characterized by transmission electron microscope ( TEM), energydispersive spectrometer ( EDS) and computer color magic image analysis system (CMIAS). A methylthiazolyl tetrazolium (MTT) assay and a flow cytometry (FCM) assay were performed to examine theantitumor effect of As_2O_3 nanoparticlesat various concentrations(1, 2, 5, 10 mumol/L). We alsocompared the antitumor effect of As_2O_3 nanoparticles with that of As_2O_3 solution. The averagediameters of two kinds of As_2O_3 nanoparticles prepared were about 80 nm and 40 nm. It wasidentified that the prepared nanoparticles were As_2O_3 and there were no other components by EDS.After 48 h of treatment with As_2O_3 nanoparticles, the survival ratio of cells was significantlylower than that of the As2O3 solution with the same concentration(P < 0. 05). Experimental resultsdemonstrate that by sol-gel method As_2O_3 can be prepared into nanoparticles. As_2O_3 nanoparticlescan produce a better cytotoxic effect on tumor cells than the As_2O_3 solution.研究了As2O3纳米粒的制备方法、表征及其抗肝癌细胞的作用.采用溶胶凝胶法制备As2O3纳米粒,并用透射电镜、能谱仪和图像分析仪等方法对其进行表征及特性检测.通过MTT法和流式细胞仪法研究了不同浓度As2O3纳米粒对人肝癌细胞株的影响,并与传统的As2O3溶液进行了比较.实验中制备了2种粒径大小的As2O3纳米粒子,其平均直径分别为80nm和40nm,通过能谱仪的测试证实所制备的纳米粒为As2O3,且无其他成分.体外细胞实验发现,As2O3纳米粒处理细胞48h后,人肝癌细胞的存活率明显低于同浓度的As2O3溶液处理组(P<0 05).研究结果显示,通过溶胶凝胶法可将As2O3制备成纳米粒子;体外细胞实验表明,与传统的As2O3溶液相比,As2O3 纳米粒子可对肿瘤细胞产生更强细胞毒作用.

关 键 词：NANOTECHNOLOGY arsenic trioxide liver cancer 

分 类 号：R73-36[医药卫生—肿瘤]