Isolation and Characterization of 44.6 kDa Protein from Schistosoma japonicum Male Worm  

作　　者：陈欲晓 易新元 曾宪芳 袁仕善 张顺科 Larry MCREYNOLDS 

机构地区：[1]Department of Immunology, Xiangya School of Medicine, Central South University, Changsha 410078, China [2]Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China [3]Molecular Parasitology Group New England Biolabs Inc.,Beverly,MA0191 [4]LMcreynolds,USA

出　　处：《Journal of Microbiology and Immunology》2004年第2期136-139,共4页中华微生物学和免疫学（英文版）

基　　金：This work was supported by WHO TDR (No.980268)

摘　　要：Soluble male worm antigen of Schistosoma japonicum ( Sj ) was investigated for development of new vaccine candidate. SDS-PAGE and Western blotting were performed to compare the difference between soluble antigens from worms of different sex. Mice vaccination with the testing purified protein was followed by Sj cercariae challenge to detect the protective effect against Sj . Sixteen bands were seen for the soluble male worm antigen and 12 for the female worm. In addition, a distinct band of 44.6 kDa from the male worm antigen was observed, and its antigenicity was demonstrated by Western blotting. This 44.6 kDa protein could induce significant worm and egg reduction rate in mice (39.31%, 41.98%, P <0.001). In this study a 44.6 kDa protein was isolated and partially characterized. Its antigenicity, immunogenicity and the partial immune protection suggest its potential vaccine candidte against Sj .Soluble male worm antigen of Schistosoma japonicum ( Sj ) was investigated for development of new vaccine candidate. SDS-PAGE and Western blotting were performed to compare the difference between soluble antigens from worms of different sex. Mice vaccination with the testing purified protein was followed by Sj cercariae challenge to detect the protective effect against Sj . Sixteen bands were seen for the soluble male worm antigen and 12 for the female worm. In addition, a distinct band of 44.6 kDa from the male worm antigen was observed, and its antigenicity was demonstrated by Western blotting. This 44.6 kDa protein could induce significant worm and egg reduction rate in mice (39.31%, 41.98%, P <0.001). In this study a 44.6 kDa protein was isolated and partially characterized. Its antigenicity, immunogenicity and the partial immune protection suggest its potential vaccine candidte against Sj .

关 键 词：Schistosoma japonicum Male adult worm ANTIGENICITY 

分 类 号：R532.21[医药卫生—内科学]