光动力疗法对血管内皮细胞和视网膜色素上皮细胞的影响  

Effect of Photodynamic Therapy on Vascular Endothelial Cells and Retinal Pigment Epithelial Cells

作　　者：金陈进[1] 周少博[1] 胡卫群[2] 葛坚[1] 江儒章[1] 陈凌燕[1] 

机构地区：[1]中山大学中山眼科中心,广东广州510060 [2]湖北孝感市中心医院眼科,湖北孝感432100

出　　处：《中山大学学报（医学科学版）》2005年第2期207-210,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技攻关计划项目(99M04702G)卫生部科技专项基金项目(WKZ-2001-1-01)

摘　　要：【目的】研究光动力疗法(PDT)在体外对视网膜色素上皮细胞和血管内皮细胞的影响。【方法】将体外培养的视网膜色素上皮(RPE)细胞和血管内皮细胞与PDT治疗年龄相关性黄斑变性时血药浓度(1.0μg/ mL)相当的光敏剂verteporfin共同孵育,根据孵育时间的不同,每种细胞分成7组,即0 min组、5 min组、15 min组、30 min组、60 min组、120min组和对照组,孵育至上述时间后,分别用光敏剂最大吸收波长的光照(689nm、功率密度为600 mW/cm2的二极管激光),能量密度为2.4 J/cm2,用MTT法检测24 h后各处理组细胞的存活率。所有实验数据均以均数±标准差的形式表述,数值为3次独立重复实验的平均值,资料统计采用方差分析和t检验。【结果】PDT后24 h,RPE细胞在0 min组、5 min组、15 min组、30min组、60min组和120 min组的细胞平均存活率分别为:0.94±0.07、0.68±0.13、0.68±0.11、0.65±0.12、0.49±0.11和0.21±0.11,细胞的存活率随与光敏剂孵育时间的延长而下降,经单因素方差分析,不同时间组的细胞存活率有显著性差异(P =0.000),SNK-q检验,5 min组、15 min组、30min组、60 min组、120 min组与0 min组比较都有统计学差异:血管内皮细胞在上述各时间组的平均存活率分别为:1.02±0.05、0.78±0.08、0.71±0.11、0.69±0.08、0.[Objective]To investigate the effect of photodynamic therapy (PDT) on retinal pigment epithelial (RPE) cells and vascular endothelial cells in vitro.[Methods] Cultured retinal pigment epithelial cells and vascular endothelial cells were incubated with verteporfin at the concentration (1.0μg/ml) that was equivalent to the initial plasma level of verteporfin in clinic therapy. According to the incubated time with verteporfm, each kind of the cells was divided into 7 groups, 0 min, 5 min, 15 min, 30 min, 60 min, 120 min, and control groups. Cells were illuminated with the light of 689nm, the maximum wavelength of absorption of verteporfin. After incubating above time, the cell viability was assessed with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltertrazoliumbromide (MTT) 24 h after PDT. The values were expressed in the figures as mean±standard deviation of the mean and they were the average of three independent experiments run in triplicate, the data were analyzed with analysis of variance (ANOVA) and student's t test. [Results] The viability of RPE cells in the groups of 0 min, 5 min, 15 min, 30 min, 60 min, and 120 min were 0.94±0.07, 0.68±0.13, 0.68±0.11, 0.65±0.12, 0.49±0.11, and 0.21±0.11, respectively. The viability of the vascular endothelial cells in the corresponding groups were 1.02±0.05, 0.78±0.08, 0.71±0.11, 0.69±0.08, 0.62±0.09, and 0.23±0.11, respectively. For both kinds of cell, there were significant differences in the cell viability among the different time groups using one-way-ANOVA,P=0.000; The cell viability was increased with the increase of incubated time with verteporfin in both kinds of cell;Differences in cell viability between 0 min group and the other groups were statistically significant using SNK-q test. However, there was no significant difference between the same time groups of the two kinds of cell in the cell viability using t test. [Conclusions] PDT has a quick killing effect on both vascular endothelial cells and RPE cells; they have equal sensitivity to PDT in vitro.

关 键 词：光动力疗法 色素上皮 血管内皮 细胞活力 视网膜 

分 类 号：R774.5[医药卫生—眼科]