刺梨果实半乳糖内酯脱氢酶基因cDNA片段的克隆及在不同器官的表达  

Cloning of cDNA Fragment of L-Galactono-1,4-Lactone Dehydrogenase and Its Expression in Different Organs of Rosa roxburghii Tratt

作　　者：安华明[1] 陈力耕[1] 樊卫国[2] 

机构地区：[1]浙江大学园艺系,杭州310029 [2]贵州大学园艺系,贵阳550025

出　　处：《中国农业科学》2005年第3期571-575,共5页Scientia Agricultura Sinica

基　　金：贵州省自然科学基金资助项目[黔科合计字(2003)3019]

摘　　要：根据 GenBank 中登录的拟南芥、花椰菜、甘薯和草莓等植物的半乳糖内酯脱氢酶基因(GalLDH)保守序列设计引物,采用 RT-PCR 法,从刺梨果实中扩增出该基因的 cDNA 片段 855 bp 并测序。序列分析表明,刺梨 GalLDH与草莓 GalLDH 的相似性达 92%,与其它植物同源基因的相似性也在 80%左右;该片段编码 285 个氨基酸,推导的氨基酸序列与其它植物同源基因的相似性高达 75%～87%。Northern 杂交分析表明,该基因在刺梨不同器官中的表达差异明显:在果实内有很高的表达水平,叶内较弱,而在茎和根内只能检测到微弱的信号。这种差异与不同器官中维生素 C 的积累量高度一致。bp cDNA encoding L-galactono-1,4-lactone dehydrogenase (GalLDH) fragment was cloned from fruit of Rosa. roxburghii Tratt by the method of RT-PCR on the basis of the homologous genes of Arabidopsis thaliana, cauliflower, sweet potato, strawberry, etc. in GenBank. Sequensing analysis shows that 79%-92% identity in nucleotide sequence and 75%-87% identity in amino acid sequence to that of strawberry and Arabidopsis thaliana, etc. was observed. Northern blot showed that the expression of GalLDH was significantly different in different organs. The transcription level of GalLDH in fruit was significantly higher than that in leaf, stem and root, respectively. Furthermore, this expression mode was highly correlated with AsA levels.

关 键 词：梨果实 刺梨 器官 草莓 脱氢酶 内酯 酶基因 半乳糖 LDH CDNA片段 

分 类 号：S661.2[农业科学—果树学] S668.4[农业科学—园艺学]