中华蜜蜂mrjp3基因cDNA的克隆及序列分析  

Cloning and Sequence Analysis of cDNA Encoding mrjp3 of Apis cerana cerana

作　　者：苏松坤[1] 陈盛禄[1] 钟伯雄[1] 郑火青[1] Stefan Albert 

机构地区：[1]浙江大学动物科学学院 [2]Institute of Medical Radiation and Cell Research,Würzburg 97078 Germany

出　　处：《中国农业科学》2005年第3期612-618,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目(30200206);浙江省自然科学基金资助项目(302113)

摘　　要：以东方蜜蜂(Apis cerana)mrjp3 基因部分片段为探针筛选中华蜜蜂(Apis cerana cerana,简称中蜂)8 日龄工蜂头部 cDNA 文库,得到 120 个阳性克隆。对这些阳性克隆进行进一步的筛选和序列测定,获得含有中蜂 MRJP3 (AccMRJP3)cDNA 的阳性克隆,对阳性克隆的插入片段进行测序,得到 AccMRJP3 的 cDNA 序列全长。中蜂 MRJP3 的 cDNA 全长为 1 987 bp(包括 10 bp 长的 poly A 尾巴),包含一个长 1 779 bp,编码 593 个氨基酸的ORF,在 5’端和 3’端各有一个长 46 bp 和 160 bp 的非编码区。类似于西方蜜蜂(Apis mellifera)和大蜜蜂(Apisdorsata),由 AccMRJP3 cDNA 编码的氨基酸序列也存在一个长片段重复区。但比较结果显示中蜂、西方蜜蜂、大蜜蜂 MRJP3 重复区之间存在一定差异。By screening the worker(Apis cerana cerana)heads cDNA library, using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. The clone containing AccMRJP3 cDNA was selected. Based on the sequencing of the inserts of the positive clone, a sequence of AccMRJP3 cDNA which is 1 887 bp long including a poly(A) tail was obtained. The AccMRJP3 cDNA encompassed an open-reading frame (ORF) with 1 779 bp encoding 593 amino acids. The un-translated regions (UTR) of the 5′end and 3′end are 46 bp and 160 bp long, respectively. Similar to AmMRJP3 and AdMRJP3, the putative AccMRJP3 also has a repetitive region. The comparison of the repetitive region of AccMRJP3, AmMRJP3 and AdMRJP3 shows some differences between them.

关 键 词：中蜂 中华蜜蜂 西方蜜蜂 东方蜜蜂 工蜂 日龄 尾巴 阳性克隆 基因CDNA 片段 

分 类 号：S891[农业科学—特种经济动物饲养]