沙立度胺对人卵巢癌裸鼠皮下移植瘤血管生成的影响  

Study of thalidomide on the growth and angiogenesis of ovary cancer SKOV3 transplanted subcutaneously in nude mice

作　　者：李文 彭芝兰[2] 曹泽毅[2] 

机构地区：[1]解放军第四五二医院妇产科,成都610061 [2]四川大学华西第二医院妇产科

出　　处：《中华妇产科杂志》2005年第3期186-189,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探讨沙立度胺(Thd;商品名:反应停)对人卵巢癌裸鼠皮下移植瘤生长及血管生成的影响。方法　建立 15只人卵巢癌裸鼠皮下移植瘤模型,随机分成 3组: (1)Thd组:腹腔内注射Thd(200mg·kg-1·d-1,浓度为 62 5mg/L); (2 )Thd+环磷酰胺 (CTX)组:按上述Thd剂量,加入浓度为 100mg/L的CTX(100mg·kg-1·d-1 ),腹腔内注射; (3)生理盐水组:腹腔内注射 0 2ml生理盐水。观察各组裸鼠皮下移植瘤生长情况、裸鼠体重的变化,并采用RT PCR、酶联免疫吸附法、及Picture二步免疫组化法检测各组瘤组织中血管内皮生长因子 (VEGF)mRNA、外周血中VEGF含量及瘤组织中微血管密度(MVD)。结果　 ( 1 )Thd组、Thd+CTX组皮下移植瘤体积明显小于生理盐水组,差异有统计学意义(P<0.05)。(2)Thd组瘤组织中VEGFmRNA为 55±9,血中VEGF含量为(29±10)pg/ml;Thd+CTX组瘤组织中VEGFmRNA为 26±7,血中VEGF含量为(12 ±6)pg/ml;生理盐水组瘤组织中VEGFmRNA为 79±7,血中VEGF含量为(71±16)pg/ml。Thd组、Thd+CTX组瘤组织中VEGFmRNA和血中VEGF含量分别与生理盐水组比较,差异均有统计学意义 (P<0.01)。(3)Thd组MVD计数为 (12.6±3.3)条,Thd+CTX组为 (10.6±1.9)条,均明显低于生理盐水组的(19 3±2 8)条(P<0 01)。Objective To study the effect of thalidomide(Thd) used alone and in combination with cytoxan (CTX) on the growth and angiogenesis of human ovarian cancer transplanted subcutaneously in nude mice. Methods Human ovarian cancer model transplanted subcutaneously in nude mice was established, and divided into 3 groups: control group, Thd group, and Thd+CTX group.Tumor volume and weight,vascular endothelial growth factor(VEGF) mRNA, VEGF protein, microvascular density(MVD) were detected. The level of VEGF mRNA in tumor tissue was determined by relative quantative reverse transcription polymerase chain reaction. VEGF protein level in serum was determined by enzyme-linked immunosorbent assay (ELISA). MVD was calculated by immunohistochemistry. Results (1)Tumor volumes in Thd group and Thd+CTX group were smaller than those in control group(P<0.05).(2)Expression of VEGF mRNA level in Thd group (55±9) and Thd+CTX group (26±7) was significantly lower than that in control group (79±7, P<0.01).Serum VEGF level in Thd group, [(29±10) pg/ml] and Thd+CTX group [(12±6) pg/ml] was significantly lower than that in control group, [(71±16) pg/ml, P<0.01].(3) MVD in Thd group (12.6±3.3) and Thd+CTX group (10.6±1.9) was significantly smaller than that in control group(19.3±2.8,P<0.01). Conclusions Thalidomide can inhibit the growth and angiogenesis of human ovarian cancer transplanted subcutaneously in nude mice. Treatment with thalidomide is a potentially useful antitumor therapy for ovarian cancer.

关 键 词：VEGF 瘤组织 CTX 裸鼠皮下移植瘤 生理盐水 人卵巢癌 腹腔内注射 RNA RT-PCR 生长 

分 类 号：R737.31[医药卫生—肿瘤]