SARS恢复期患者血液与排泄物中SARS-CoV RNA的检测  

Study on the RNA of severe acute respiratory syndrome(SARS)-associated coronavirus in the blood and excretion of convalescent patients with SARS

作　　者：常昭瑞[1] 杨仁全[2] 王彦斌[1] 任丽丽[1] 王敏[1] 杨耀武[1] 郭丽[1] 晁彦公[3] 曲成毅[4] 王健伟[1] 洪涛[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052 [2]南京农业大学 [3]清华大学附属北京酒仙桥医院 [4]山西医科大学公共卫生学院

出　　处：《中华流行病学杂志》2005年第3期201-203,共3页Chinese Journal of Epidemiology

基　　金：国家"863"计划专项基金资助项目(2003AA208403)

摘　　要：目的　应用巢式逆转录聚合酶链反应(RT-PCR)检测严重急性呼吸综合征(SARS)恢复期患者血液与排泄物中的病毒 RNA。方法　对连续 3 次收集的 23 例确诊 SARS恢复期(病程≥21天)患者的血、尿、痰、粪便标本进行核酸提取,设计特异性内外引物,使用巢式RT PCR的方法进行病毒RNA检测。结果　通过巢式RT PCR,共检测到6份阳性标本,其中粪便标本中检测到 4 份,检出率为5.8%;痰标本中检测到 2 份,检出率为2.9%。在尿液和血液标本中未检测到病毒 RNA。结论个别SARS恢复期患者排泄物中有SARS病毒RNA存在,因此对恢复期患者的排泄物应慎重处理,对其引起SARS的再次传播的危险性需要进一步评估。Objective To examine the RNA of severe acute respiratory syndrome-associated coronavirus(SARS-CoV) in the blood and excretion of convalescent patient with SARS for prevention and treatment of the disease. Methods A total of 276 samples, including plasma, urine, feces and sputum, obtained from 23 convalescent patients with SARS were studied at 3 time-points at least 21 days after the onset of symptoms. RNA was extracted and nested reverse transcription-polymerase chain reaction(RT-PCR) was carried out using SARS-CoV specific primers. Results Among the 276 samples, SARS-CoV RNA was detected in 6 cases((38.8)%) by nested RT-PCR. The positive rates of SARS-CoV RNA was (5.8)% in feces and (2.9)% in sputum samples but SARS-CoV RNA was not detectable in plasma and urine of all the cases. Conclusion The existence of SARS-CoV RNA in the excretion of some convalescent patients with SARS showed that the excretion from these patients should be carefully treated whilthe re-transmission of SARS by which, should be further studied.

关 键 词：患者 SARS恢复期 RNA 血液 粪便标本 RT—PCR SARS-COV 排泄物 病毒 引物 

分 类 号：R511.9[医药卫生—内科学]