多重PCR技术在植物生物学研究中的应用  

Application of Multiplex PCR to Studies on Plant Biology

作　　者：刘正斌[1] 高庆荣[1] 王瑞霞[1] 乔晓琳[1] 邱新民[1] 

机构地区：[1]山东农业大学农学院,泰安271018

出　　处：《分子植物育种》2005年第2期261-268,共8页Molecular Plant Breeding

基　　金：山东省自然科学基金(Y2002D08);国家863发展计划(2002A207004)资助。

摘　　要：多重PCR是近几年兴起的一项新技术,它高效快捷﹑高度特异敏感﹑能够降低实验成本很适宜于大样本的植物生物学研究。本文较为详细地介绍了多重PCR的概念、原理及其在国内外植物种质纯度鉴定、病虫害检测和植物分子育种中的应用现状,并对其应用前景作了展望。Multiplex PCR is a newly developed technology, which is highly efficient, rapid, specific and sensitive, meanwhile which can reduce the cost of experiment. So multiplex PCR is extremely suitable for studies on the plant biology. In this article, conception and principles of multiplex PCR are introduced. In the meanwhile, its applications to identify the pureness of germplasm of some plants, to detect plant diseases and insect pests and to plant molecule breedings are all given a presentation. Future of multiplex PCR is viewed in the last.

关 键 词：多重PCR技术 种质 分子育种 纯度鉴定 病虫害 植物生物学 应用现状 物种 敏感 

分 类 号：Q94-33[生物学—植物学]