螺内酯对肾血管性高血压大鼠左室重构的影响  

Effects of Spironolactone on the Left Ventricular Remodeling In Renovascular Hypertension Rats

作　　者：王圣[1] 李新明[1] 刘宇飞[2] 丁彦春[2] 夏稻子[2] 曲鹏[2] 

机构地区：[1]海南省人民医院心内科,海南海口570311 [2]大连医科大学附属第二医院心内科,大连116024

出　　处：《海南医学》2005年第4期126-128,共3页Hainan Medical Journal

摘　　要：目的 探讨在高血压左室肥厚(LVI-H)形成过程中螺内酯对肾血管性高血压大鼠心肌纤维化以及左室 重构的影响。方法 采用两肾一夹(2K1C)法在SD大鼠中建立肾血管性高血压大鼠模型,随机分为非药物干预 组(N组)、螺内酯组(螺内酯50 mg·kg-1·d-1灌胃,S组),并以假手术组大鼠(C组)为对照。采用心脏超声观察各 组大鼠心脏结构和功能的变化;放射免疫法检测各组大鼠心肌组织中血管紧张素Ⅱ、醛固酮的浓度;Woessner法 测定心肌组织中胶原含量。结果 治疗8周后S组大鼠与N组相比血压、LVEDD、LVPWD、RWT、LVM、LVMI、 MESS以及mFS等指标较N组稍有下降,但无显著差异(P>0.05)。S组心肌组织中AngⅡ(5.86±3.50 ng／g vs 8.10±2.64 ng／g;P<0.05)、心肌胶原浓度(4.49±0.98 mg／100mg vs 5.46±1.12 mg／100mg;P<0.01)、心肌内血管周 围胶原面积(24.68±4.62％vs 35.28±5.80％;P<0.01)、胶原容积分数(2.18±0.20％vs 3.26±0.28％;P<0.01)均明显 低于N组(P<0.05)。结论 (1)2K1C法可以在SD大鼠中产生明确的肾血管性高血压、LVH,心肌纤维化以及 心功能减退。(2)螺内酯可以抑制肾血管性高血压大鼠心肌纤维化的形成,但对血压以及心脏结构和功能的改变 影响较小。Objective To investigate the effects of spironolactone on the myocardial fibrosis and left ventricular remodeling during the development of left ventricular hypertrophy (LVH) in renovascular hypertension rats. Methods Two-kidney, one-clip (2K1C) renovascular hypertension was induced in Sprague-Dawley rats. The rats were randomized to non-intervention group (N group), spironolactone group (S group) that gorged spironolactone 50mg·kg-1-d-1. The sham-operated rats served as control group (C group). The changes of heart structure and function in all groups were observed with echocardiogram. The concentration of angiotensin Ⅱ (Ang Ⅱ) and aldosterone (ALD) in left ventricle were assessed by radioimmunoassay. The Woessner's method was used to estimate the collagen content in left ventricle. Results After 8 weeks treatment, blood pressure, LVEDD, LVPWD, RWT , LVM, LVM1, MESS and mFS in S group decrease slightly compared with N group (P>0.05). The concentration of Ang Ⅱ in S group is much lower than in N group (5.86±3.50 ng/g vs 8.10±2.64 ng/g; P<0.05). The collagen content (4.49±0.98 mg/100mg vs 5.46±1.12 mg/100mg; P<0.01), PVCA(24.68±4.62% vs 35.28±5.80%; P<0.01) and CVF (2.18±0.20% vs 3.26±0.28%; P<0.01) in S group are much lower than in N group. Conclusion: (1) Renovascular hypertension, LVH, myocardial fibrosis and left ventricular dysfunction can be developed in 2K1C rats. (2) Spironolactone is very effective in reducing myocardial fibrosis, but has weak effects on blood pressure and suppressing the changes of structure and function of left ventricular in renovascular hypertension rats.

关 键 词：螺内酯 高血压 左室重构 心肌纤维化 

分 类 号：R-33[医药卫生]