胃癌组织中PTEN,VEGF,MMP-9的表达及相关性研究  

A study of the correlation and expression of PTEN ,VEGF and MMP-9 in human gastric carcinoma

作　　者：贺荣芳[1] 胡忠良[1] 沈明[1] 文继舫[1] 

机构地区：[1]中南大学湘雅医学院病理学系,湖南长沙410078

出　　处：《中国普通外科杂志》2005年第3期173-177,共5页China Journal of General Surgery

摘　　要：目的　探讨胃癌中PTEN,VEGF,MMP-9的表达及其与胃癌生物学行为的关系。方法　应用免疫组织化学SP法检测 7 1例胃癌组织及 3 7 例远癌胃黏膜中PTEN,VEGF,MMP-9 的表达。结果　胃癌组织中PTEN蛋白表达 ( 7 1. 8% )显著低于远癌胃黏膜的表达 ( 1 0 0% ) (P < 0. 0 1 ) , 且与肿瘤的组织分化程度、浸润深度及淋巴结转移呈负相关 (均P< 0. 0 5 ),而与年龄、性别等无关 (均P> 0. 0 5 ) 。VEGF在胃癌中的表达 ( 6 2. 0% ) 显著高于远癌胃黏膜的表达 ( 2 9. 7% ) (P < 0. 0 1 ) ,且与淋巴结转移呈正相关 (P< 0. 0 1 ) ,而与年龄、性别、组织分化程度及肿块浸润深度无关 (均P> 0. 0 5 )。MMP-9在胃癌中的表达 ( 6 9% ) 显著高于远癌胃黏膜的表达 ( 4 0. 5% ) (P < 0. 0 1 ),且与淋巴结转移呈正相关 (P< 0. 0 5 ) , 而与年龄、性别、组织分化程度及癌组织浸润深度无关 (均P> 0. 0 5 )。PTEN在胃癌中的表达与VEGF和MMP-9呈负相关 (P < 0. 0 5 )。结论　胃癌中PTEN蛋白表达降低,其表达与肿瘤的组织分化程度、浸润深度及淋巴结转移密切相关,且与VEGF,MMP-9表达呈负相关。联合检测PTEN,VEGF,MMP-9有助于提高胃癌侵袭转移能力的评估,对胃癌的预后判断具有重要的临床意义。Objective To identify the expression of PTEN ,vascular endothelial growth factor(VEGF)and (matrix) metalloproteinase 9(MMP-9) in gastric carcinoma and its relationship to the biological behavior of (gastric) carcinoma. Methods The expression of PTEN ,VEGF and MMP-9 in 71 cases of gastric carcinoma tissue and 37 cases of gastric mucosa distant from carcinoma were detected by streptavidin peroxidase (immunohistochemistry). Results The expression of PTEN in gastric carcinoma tissues(71.8%) was (significantly) lower than its expression in gastric mucosa distant from carcinoma(100%)(P<0.01),and expression of PTEN was inversely correlated with the histologic differentiation of the tumor,depth of tumor (infiltration) and lymph node metastasis(P<0.05,in all parameters),but was not related to age or sex of the patient(P>0.05).The expression of VEGF in gastric carcinoma(62.5%) was sigificantly higher than in gastric mucosa distant from carcinoma(29.7%)(P<0.01),The expression of VEGF was (positively) correlated with lymph node metastasis(P<0.01),but was not (related) to age,sex of the patient,and the degree of (histological) differentition or depth of tumor infiltration(P>0.05).The expression of MMP-9 in gastric (carcinoma)(69%) was significantly higer than in gastric mucosa distant form carcinoma(40.5%)(P<(0.01)) and was positively correlated with lymph node metastasis(P<0.05),but not (related) to age,sex of the patient,and the degree of histological differentiation or depth of tumor infiltration(P>0.05).The expression of PTEN in gastric carcinoma was inversely correlated with expression of VEGF and MMP-9(P<0.05). Conclusions Decreased expression of PTEN was found in gastric carcinoma and (significantly) correlated with histologic (differentiation)、 depth of infiltration and lymph node metastasis of gastric carcinoma.Also,the expression of PTEN was inversely correlated with the expression of VEGF and MMP-9 ingastric carcinoma. The combined (detection) of PTEN,VEGF and MMP-9 may be of important clinical value toevalu

关 键 词：胃肿瘤/病理学 癌基因蚩白表达 PTEN VEGF MMP一9 

分 类 号：R735.2[医药卫生—肿瘤] R730.231[医药卫生—临床医学]