VEGF对体外培养的人脐静脉内皮细胞Ets-1的诱导表达  

VEGF-induced expressions of Ets-1 of cultured human umbilical endothelial cells in vitro

作　　者：石英爱[1] 李玉林[1] 赵强[2] 吴珊[1] 何旭[1] 

机构地区：[1]吉林大学基础医学院病理生物学教育部重点研究室,吉林长春130021 [2]吉林大学第一医院儿外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2005年第2期166-168,共3页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题 (C0 30 2 0 3)

摘　　要：目的 :探讨血管内皮细胞生长因子 (VEGF)对体外培养的人脐静脉内皮细胞 (HU VECs)中转录因子 Ets- 1的诱导表达。方法 :原代培养获得 HUVECs;应用细胞免疫化学染色技术及形态计量分析系统 Image- ProPlus(IPP) ,检测 VEGF对培养的内皮细胞 Ets- 1蛋白的诱导表达作用并对相关指标进行半定量分析。结果 :VEGF(10 μg· L- 1 )可诱导内皮细胞 Ets- 1蛋白的短时表达升高 (P<0 .0 1) ,即随时间的延长 ,Ets- 1表达也逐渐增强 ,在 10 min时达高峰 ,30 min后 Ets- 1表达逐渐降低到无 VEGF刺激的水平。结论 :VEGF可诱导人脐静脉内皮细胞中 Ets- 1蛋白的短时表达增加。Objective To study the VEGF-induced expressions of Ets-1 of human umbilical vein endothelial cells(HUVECs) cultured in vitro. Methods The HUVECs were cultured and obtained. The immunocytochemical stain and Image-Pro Plus (IPP) analysis system were used to determine the VEGF-induced expressions of Ets-1 in the cultured endothelial cells. The relative indexes were analyzed with semi-quantitative technique. Results The VEGF (10 μg·L~ -1) induced the short-term expressions of Ets-1 in HUVECs (P<0.01), and Ets-1 expressions increased with the prolongation of time. The expressions of Ets-1 protein in HUVECs reached a peak at 10 min. After 30 min, the intensity of the expressions of Ets-1 decreased gradually to the level of no VEGF-induced. Conclusion The VEGF can induce the short-term expressions increase of Ets-1 of HUVECs.

关 键 词：内皮生长因子 转录因子 内皮 血管/细胞学 脐静脉 细胞 培养的 

分 类 号：R730.1[医药卫生—肿瘤]