应用PCR检测单核细胞增多症李氏菌的研究  

SPECIES-SPECIFIC DETECTION OF LISTERIA MONOCYTOGENES BY USING THE POLYMERASE CHAIN REACTIN

作　　者：杨百亮[1] 聂向庭[1] 马海利[1] 杨兴周[1] 周志江[1] 徐克诚[1] 张让堂 刘纯杰[1] 

机构地区：[1]山西农业大学动医系,解放军兽医大学卫检系

出　　处：《畜牧兽医学报》1994年第4期353-358,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山西省青年科学基金

摘　　要：以一对位于单核细胞增多症李氏菌β－溶血素基因内的２６－ｂｐ寡核苷酸为引物，用ＰＣＲ对单核细胞增多症李氏菌进行了检测，结果所有株单核细胞增多症李氏菌均扩增出１８６－ｂＰ的特异条带，其它种李氏菌和细菌均呈阴性反应。本方法可测出模拟肉样中少至９２个细菌，模拟奶样中少至１８个细菌，其特异性和敏感性不受其它污染杂菌ＤＮＡ的影响。对市售猪肉和牛奶的检测结果表明，ＰＣＲ法优于传统的分离培养法，具有快速、简便和敏感的优点。The polymerase chain reaction was used to detect virulent Listeria monocytogenes.A 186-bp DNA fragment was shown to be specifically amplified in L,monocytogenes butnot in other species of Listeria or in a number of other gram-positive and gram-nega-tive bacteria. As few as 92 bacteria in the simulated pork sample and l8 bacteria inthe simulated milk sample could be detected.The presence of nonlisterial bacteria hasno effect on the sensitivity and specificity of the method.The overall utility of thepolymerase chain reaction was also assessed by direct testing of pork and milk samplescollected in the market.

关 键 词：PCR 单核细胞 增多症 李氏菌 检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]