金地鼠颊囊癌变过程中p65蛋白的表达研究  

Expression of p65 in the carcinogenesis of hamster buccal mucosa induced by DMBA

作　　者：莫珩[1] 高文信[1] 王莉[1] 冯小东[1] 

机构地区：[1]吉林大学口腔医学院口腔粘膜科,长春130041

出　　处：《现代口腔医学杂志》2005年第2期165-167,共3页Journal of Modern Stomatology

摘　　要：目的探讨核转录因子-κB家族成员中的一个重要亚基p6 5在DMBA诱导的金地鼠颊囊鳞状细胞癌发生过程中的表达及意义。方法建立金地鼠颊囊癌变的动物模型。采用Westernblot法检测12组配对金地鼠正常颊粘膜、上皮单纯增生、上皮异常增生和鳞状细胞癌组织所提取的核蛋白中p6 5的表达差异。结果在正常颊粘膜和上皮单纯增生组织中未见明显的p6 5表达,且两者相比无显著性差异(P >0 .0 5 )。随着上皮异常增生的出现,p6 5表达增强,较正常组和单纯增生组均有显著性差异(P <0 .0 1)。在鳞状细胞癌组织中,p6 5的表达进一步增强,与正常组和异常增生组相比均有显著性差异(P <0 .0 1)。结论p6 5在金地鼠颊囊鳞状细胞癌发生、发展过程中被激活。P6 5在金地鼠颊囊癌变过程中的表达异常,尤其是在上皮异常增生阶段的表达水平上调,可能是口腔粘膜上皮癌变过程中的一个早期事件。Objective To study the expression and significance of p65, an important subtype of nuclear transcription factor κB (NF-κB) in the malignant transformation of hamster buccal mucosa.Methods The malignant transformation of hamster buccal mucosa induced by DMBA (~0.5% ) was performed to establish the animal model. Twelve paired specimens, including normal buccal mucosa, epithelial hyperplasia, epithelial dysplasia, and squamous cell carcinoma (SCC) were subjected to Western blot for analysis against p65.Results The expression of p65 was not found in normal buccal epithelia or hyperplastic epithelia and no significant difference was found between the two groups. With the dysplastic epithelia, as the severity of dysplasia increased, the expression of p65 gradually increased and this was compared with normal buccal epithelia and hyperplastic epithelia and found to be significant (P<~0.01 ). The expression of p65 was much higher in SCC than in normal buccal mucosa or epithelial dysplasia (P<~0.01 ).Conclusion NF-κB p65 is strongly activated in the malignant transformation of hamster buccal mucosa. The abnormal expression of p65, especially the up regulation of p65 in dysplasia may be an early event during oral carcinogenesis.

关 键 词：地鼠 表达水平 癌变过程 颊囊癌 正常 上皮异常增生 鳞状细胞癌 表达差异 核蛋白 粘膜 

分 类 号：R739.8[医药卫生—肿瘤]