丙戊酸钠对大鼠坐骨神经修复与再生的影响  

Effect of sodium valproate on sciatic nerve repair and regeneration in rats

作　　者：刘世清[1] 吴飞[1] 饶婷[2] 张忠礼[1] 梅红军[1] 

机构地区：[1]武汉大学人民医院骨外科,湖北省武汉市430060 [2]武汉大学人民医院泌尿外科,湖北省武汉市430060

出　　处：《中国临床康复》2005年第9期26-28,i001,共4页Chinese Journal of Clinical Rehabilitation

基　　金：湖北省攻关课题(982P1514)~~

摘　　要：目的:通过对坐骨神经损伤模型的药物实验研究,观察丙戊酸钠对大鼠周围神经损伤的修复作用。方法:实验于2004-07/11在武汉大学医学院动物实验中心进行。采用清洁级SD大鼠36只,行右坐骨神经横形切断后即刻原位吻合术,制成周围神经损伤模型后,随机分为丙戊酸钠组、对照组、维生素组。分别在给药后4,8,12周以坐骨神经功能指数(sciaticnervefunctionindex,SFI)、坐骨神经传导速度(nerveconductionvelocity,NCV)和坐骨神经轴突计数(sciaticnerveaxoncounting,NAC)为观测指标,判断神经功能的修复情况。结果:①术后4,8,12周坐骨神经功能指数检测结果:丙戊酸钠组分别为-74.75±1.55,-67.41±2.21,-55.29±0.82,明显好于对照组-84.60±2.00,-79.43±0.72,-73.41±0.94和维生素组-77.15±0.90,-73.55±0.92,-63.48±0.57(P均<0.05)。②再生神经运动诱发电位潜伏期检测结果:丙戊酸钠组分别为(2.19±0.13),(1.97±0.28),(1.61±0.73)ms,明显好于对照组(3.10±2.11),(2.62±1.79),(2.58±0.16)ms和维生素组(2.64±1.94),(2.59±0.67),(2.54±0.09)ms(P均<0.05)。③术后12周单位视野有髓神经纤维计数结果:丙戊酸钠用药组(152.33±7.88)个/视野优于对照组(116.08±12.63)个/视野和维生素组(136.42±11.41)个/视野(P均<0.AIM:To observe the effects of sodium valproate in the repair of rat sciatic nerve injury through the drug experiment of the model of sciatic nerve injury.METHODS:The experiment was conducted in the laboratory for animals in the Medical College of Wuhan University from July to November 2004.The models of peripheral nerve injury were made by crossly cutting right sciatic nerves in 36 clean SD rats followed by neurorrhaphy.Then all injured rats were randomly divided into 3 equal groups:sodium valproate group,control group and vitamin group.After 4,8 and 12 weeks,the sciatic nerve function index(SFI),nerve conduction velocity(NCV) and sciatic nerve axon counting(NAC) were measured to verify the repair of nerve function.RESULTS:① At 4,8 and 12 weeks after operation,the SFI of sodium valproate group(- 74.75± 1.55,- 67.41± 2.21,- 55.29± 0.82 respectively) was higher than that of control group(- 84.60± 2.00,- 79.43± 0.72, - 73.41± 0.94) and significantly higher than that of vitamin group(- 77.15± 0.90,- 73.55± 0.92,- 63.48± 0.57)(both P< 0.05).② The evoked potential latency of the regenerated nerve motion in sodium valproate group[(2.19± 0.13),(1.97± 0.28),(1.61± 0.73) ms respectively] was significantly shorter than that in control group[(3.10± 2.11),(2.62± 1.79),(2.58± 0.16) ms] and vitamin group[(2.64± 1.94),(2.59± 0.67),(2.54± 0.09) ms](both P< 0.05).③ At 12 weeks after operation,the average number of myelinated nerve fibers per sight in sodium valproate group(152.33± 7.88) was more than that in control group(116.08± 12.63) and vitamin group(136.42± 11.41)(both P< 0.05).CONCLUSION:Sodium valproate is helpful to enhance the recovery of nerve function in rats after peripheral nerve injury.

关 键 词：周围神经/损伤 神经再生 丙戊酸 

分 类 号：R74[医药卫生—神经病学与精神病学]