血小板活化因子受体拮抗剂的神经保护作用  

Neuroprotective role of antagonist of platelet active factor receptor

作　　者：王岚[1] 徐运[1] 黄嵘[1] 王翀[1] 朱琳[1] 管得宁[1] 王晓云[1] 朱文斌[1] 

机构地区：[1]南京大学医学院附属鼓楼医院神经内科,江苏省南京市210008

出　　处：《中国临床康复》2005年第9期62-63,i003,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金项目(30470612);南京市医学重点课题(zkx0412)~~

摘　　要：目的:探讨血小板活化因子(platelet-activatingfactor,PAF)所致的神经元损伤是否通过PAF受体而起作用,PAF受体拮抗剂对神经元损伤是否具有保护作用。方法:实验于2003-05/2004-05在南京大学医学院附属鼓楼医院神经科研究室和美国约翰霍普金斯大学Dr.Tao实验室完成。使用原代野生型小鼠皮质神经元细胞培养系统;不同剂量PAF处理神经元细胞24h,并经PAF拮抗剂5μmol/LBN52021预处理;碘化物/钙黄绿素染色检测细胞凋亡,MTT法测定神经元生存能力。结果:0.3和0.6μmol/LPAF明显增加神经元细胞死亡率,0.01和0.10μmol/L增加死亡率不明显;PAF受体拮抗剂5μmol/LBN52021明显地抑制0.3μmol/LPAF所致的神经毒性。结论:PAF介导的神经元损伤是通过其受体而作用的,PAF受体拮抗剂对神经元损伤具有保护作用。AIM:To investigate whether platelet- activating factor(PAF) receptors affect neuron damage induced by PAF,and study whether the protective function of antagonist of PAF receptor on neuron damage exists.METHODS:The experiment was finished in the Department of Neurology,Drum Tower Hospital,Medical College of Nanjing University and Dr.Tao Laboratory of the Johns Hopkins University between May 2003 and May 2004.Primary Wild- type mice cortex neurons culture was used.Neuron cells were treated with different dosages of PAF for 24 hours,and pretreated with 5 μ mol/L BN52021.Then cells were stained with iodide or calcein to detect apoptosis.The survival ability of neurons was tested by MTT. RESULTS:Apoptosis rate of cortical neurons was significantly higher in 0.3 and 0.6 μ mol/L PAF,but it was not obviously increased in 0.01 and 0.10 μ mol/L PAF. 5 μ mol/L BN52021 could reduce the neurotoxicity induced by 0.3 μ mol/L PAF.CONCLUSION:The neuron damage induced by PAF is involved in PAF receptor,and antagonist of platelet activiting factor receptor can protect the neuron damage.

关 键 词：血小板活化因子/拮抗剂和抑制剂 神经元 细胞凋亡 

分 类 号：R74[医药卫生—神经病学与精神病学]