益肾降浊汤对高脂血症小鼠脑缺血再灌后能量代谢的影响  

Effects of yishen jiangzhuo decoction on disorder of energy metabolism induced by cerebral ischemia-reperfusion in mice with hyperlipemia

作　　者：钱涛[1] 高维娟[1] 许顺江[2] 丛斌[3] 庞桂芬[1] 

机构地区：[1]承德医学院附属医院神经外科,河北省承德市067000 [2]河北省疾病控制中心慢病科,河北省石家庄市050021 [3]河北医科大学病理生理学教研室,河北省石家庄市050017

出　　处：《中国临床康复》2005年第9期123-125,共3页Chinese Journal of Clinical Rehabilitation

基　　金：承德市科技局科技攻关基金项目(20031016)~~

摘　　要：目的:探讨能量代谢障碍在缺血性神经元损伤中的作用及益肾降浊汤对高脂血症小鼠脑缺血再灌后能量代谢的影响。方法:在造成小鼠高脂血症基础上,进行反复脑缺血再灌注,制备智能障碍模型。采用高效液相色谱法和生物化学法分别测定脑细胞能量负荷值和脑组织琥珀酸脱氢酶(succinatedehydrogenase,SDH),细胞色素氧化酶(cytochromecoxidase,CCO),Na+-K+-ATPase活性。结果:模型组小鼠脑细胞能量负荷值,脑组织SDH,CCO,Na+-K+-ATPase活性均显著低于正常对照组(P<0.01),益肾降浊汤可提高模型小鼠脑组织SDH,CCO,Na+-K+-ATPase活性及脑细胞能量负荷水平(P<0.01)。结论:益肾降浊汤可改善高脂血症小鼠脑缺血再灌后能量代谢障碍。AIM:To investigate the role of energy metabolism disorder in ischemic neuronal injury and the effects of yishen jiangzhuo decoction(YSJZD) on disorder of energy metabolism induced by cerebral ischemia and reperfusion in mice with hyperlipemia. METHODS:The model of intelligent disturbance was established by repeated cerebral ischemia- reperfusion in hyperlipemia mice.The brain energy charge was calculated, and the activities of succinate dehydrogenase(SDH), cytochrome C oxidase(CCO) and Na+ - K+ - ATPase were measured with biochemical methods and high performance liquid chromatography(HPLC). RESULTS:The brain energy charge and the activities of SDH,CCO and Na+ - K+ - ATPase in rats of model groups were obviously lower than those of control group(P< 0.01),and could be increased significantly by YSJZD (P< 0.01). CONCLUSION:YSJZD can improve the disorder of energy metabolism induced by cerebral ischemia and reperfusion in mice with hyperlipemia.

关 键 词：高脂血症/中药疗法 脑缺血/治疗 能量代谢 疾病模型 动物 

分 类 号：R743[医药卫生—神经病学与精神病学]