铝对大鼠海马CA3区诱发电位的抑制及其与胆碱能、γ-氨基丁酸系统的关系(英文)  

Inhibitive effect of aluminium on evoked potentials in hippocampal CA3 region in rats and the relationship with cholinergic and gamma-aminobutyric acid system

作　　者：胡剑峰[1] 肖鸿美[2] 李艾[2] 

机构地区：[1]湖北职业技术学院医学院,湖北省孝感市432000 [2]华中科技大学同济医学院基础医学院生理系,湖北省武汉市430000

出　　处：《中国临床康复》2005年第9期218-221,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(39270591)~~

摘　　要：背景:以往的研究表明,铝可通过影响细胞内钙稳态、降低蛋白激酶C(PKC)的活性、影响谷氨酸的释放等多种途径影响动物的学习和记忆。含铝饲料喂养的大鼠,其海马CA3区长时程增强(LTP)形成减弱。经进一步采用急性给铝的方法,将三氯化铝(AlCl3)直接微量注射到海马CA3区,观察到铝能减弱海马CA3区的诱发电位、阻抑LTP的产生,这种作用可能与铝损害了L-Arg-NO途径有关。目的:探讨铝对学习记忆的损害及与胆碱能系统和γ-氨基丁酸(GABA)系统的相关性。设计:以实验动物为研究对象,完全随机对照的研究。单位:一所大学生理系、一所职业技术学院医学院。材料:实验于2000-09/2001-04在华中科技大学同济医学院生理系神经电生理实验室完成。实验用SD大鼠68只,由华中科技大学同济医学院实验动物学部提供,普通级,体质量150～250g,雌雄不拘。干预:SD大鼠随机分为9组,分别为生理盐水组对照组6只,在其海马CA3区内先后(间隔1min)2次微量注射生理盐水各1μL;生理盐水+AlCl3组6只,在海马CA3区内先(间隔1min)微量注射生理盐水与0.5mol/LAlCl3各1μL。生理盐水+Tac组6只,在CA3区先后微量注射生理盐水与1×10-9mol/L各Tacrine1μL。生理盐水+Bic组6只,在CA3区先后微量注射生理盐水和1×10-3mol/LBicuculline各1μL。Tac+AlCl3组:预先?BACKGROUND:As indicated by previous researches, aluminium (Al) could affect learning and memory of animals through many approaches including affecting the stable status of intracellular calcium, decreasing protein kinase C(PKC) activity,and affecting the release of glutamic acid(Glu).The formation of long- term potentiation(LTP) weakens in hippocampal CA3 region of rats fed by forage containing Al.It could be found that Al would weaken evoked potential(EP) in hippocampal CA3 region and inhibit LTP formation, which might be related with the damaging effect of Al on L- Arg- NO approach through further application of acute Al administration, i.e.,AlCl3 is directly injected into hippocampal CA3 region by microinjection. OBJECTIVE:To investigate the damaging effect of Al on learning and memory,and its correlation with cholinergic system and gamma- aminobutyric acid(GABA) system.DESIGN:A completely randomized controlled verifying study based on the experimental animals.SETTING:Department of psychology in a university and the medical college of an occupational technology college.MATERIALS:The study was conducted in the Laboratory of Neuro- Electrophysiology,the Faculty of Physiology,Tongji Medical College,Huazhong University of Science and Technology between September 2000 and April 2001.Totally 68 SD rats of ordinary grade in either gender with a body mass between 150 g and 250 g were obtained from the Department of Experimental Animals of Tongji Medical College,Huazhong University of Science and Technology.INTERVENTIONS:SD rats were randomly divided into 9 groups including normal NS(NS) control group(n=6):1 μ L of NS was injected twice(1 minute interval) into hippocampal CA3 region by microinjection; NS+ AlCl3 group(n=6):1 μ L of NS and 0.5 mol/L of AlCl3 were injected(1 minute interval) into hippocampal CA3 region by microinjection;NS + Tac group(n=6):1 μ L of NS and 1× 10- 9 mol/L of Tacrine were injected in turn into hippocampal CA3 region by microinjection;NS + Bic group(n=6): 1 μ L of NS and 1 × 10- 3 mo

关 键 词：铝 诱发电位 乙酰胆碱 GABA 海马 

分 类 号：R74[医药卫生—神经病学与精神病学]