红景天对衰老小鼠脑过氧化脂质及线粒体超微结构的影响(英文)  

Effect of rhodiola on lipidperoxides and ultrastructure of mitochondria in the brain of senile mice

作　　者：张志军[1] 江文[1] 万群[1] 

机构地区：[1]解放军第四军医大学西京医院神经内科,陕西省西安市710033

出　　处：《中国临床康复》2005年第9期235-237,i006,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:衰老是生物体内各组织、器官的退行性改变,是诸多病理、生理过程综合作用的结果。红景天是一种天然药用植物,具有延缓机体衰老、防治老年病等功能,但是其确切机制仍不十分清楚。目的:探讨藏药红景天提取物(诺迪康胶囊)抗衰老作用及其机制。设计:以实验动物为研究对象,随机对照的实验研究。单位:一所军医大学医院的神经内科。材料:实验于2002-03/08在解放军第四军医大学药理学实验室完成。60只昆明种小鼠随机分为正常对照组、模型组、诺迪康1,2,4g/kg3个剂量组、维生素E阳性对照组0.1mg/kg,每组10只。干预:模型组、诺迪康组、维生素E阳性对照组均将30g/LD-半乳糖生理盐水溶液,按每日150mg/kg颈后部皮下注射,连续8周,同时,诺迪康组、维生素E阳性对照组灌胃给药,1次/d,连续8周。主要观察指标:诺迪康胶囊对衰老小鼠脑乳酸脱氢酶(lactatedehydroge-nase,LDH)活性、过氧化脂质(lipidperoxides,LPO)含量,脑过氧化物歧化酶(superoxidedismutase,SOD)活性、丙二醛(malondialdehyde,MDA)含量以及海马神经元线粒体超微结构的影响。结果:诺迪康胶囊1,2,4g/kg组均可降低脑组织LPO含量犤(30.1±2.9),(27.8±3.1),(26.9±1.9)nmol/g犦和MDA含量犤(110.2±13.5),(95.4±20.1),(90.2±16.5)nmol/g犦,与模型组犤(33.BACKGROUND:Senility is the degenerative change of every tissue and organ in b iological body,which is the result due to comprehensive actions of various patho logical and physiological processes. Rhodiola is a kind of natural medical plant ,acting on delaying organic senility,preventing and treating senile disorders,et c.But the definite mechanisms on those have been unknown yet.OBJECTIVE:To probe into the mechanism of rhodiola extract(nuodikang capsule) on anti- senility.DESIGN:Randomized, controlled experimental research based on the experimental animals.SETTING:Neurological internal department in a military hospital affiliated to a university of Chinese PLA.MATERIALS:The experiment was performed in Pathology Experiment Room of Fourth Military Medical University of Chinese PLA from March to August 2002.Sixty Kunm ing mice were randomized into normal control group, model group, 3 dosage groups of 1,2,4 g/kg of nuodikang successively and Vitamin E(Vit.E) positive control g roup 0.1 mg/kg, 10 mice in each group.INTERVENTIONS:In the model, nuodikang groups and Vit.E positive control, phys iological saline solution of D- galactose 30 g/L was applied in subcutaneous at the back of neck,150 mg/kg per day, continuously for 8 weeks.Simultaneously, in nuodikang group and Vit.E positive control,the medicine was applied by gastric perfusion, once daily,continuously for 8 weeks.MAIN OUTCOME MEASURES:Effects of nuodikang capsule on activity of lactate deh ydrogenase(LDH),the content of lipidperoxides(LPO), the activity of superoxide d ismutase(SOD) in the brain,the content of malondialdehyde(MDA) and ultrastructur e of mitochondrion in hippocampal neurons.RESULTS:In nuodikang capsule groups of 1,2,4 g/kg,the significant differences (P< 0.05) presented in LPO content in brain tissue[(30.1 ± 2.9),(27.8± 3.1), (26.9± 1.9) nmol/g] and MDA content[(110.2± 13.5),(95.4± 20.1),(90.2± 16.5) nmol/g],1 compared with the model group[(33.4± 2.2), (126.5± 17.2) nmol/g],ind icating significant differences(P< 0.05),and LD

关 键 词：抗衰老药(中药) 红景天属 过氧化脂质类 显微镜检查 电子线粒体 

分 类 号：R74[医药卫生—神经病学与精神病学]