安宫牛黄丸对脑梗死大鼠血清乳酸脱氢酶同工酶的影响(英文)  

Effect of angong niuhuang wan on serum lactate dehydrogenase isoenzymes in ra ts with cerebral infarction

作　　者：汤毅珊[1] 潘华新[1] 王培训[1] 王宁生[1] 

机构地区：[1]广州中医药大学临床药理研究所,广东省广州市510405

出　　处：《中国临床康复》2005年第9期242-243,共2页Chinese Journal of Clinical Rehabilitation

基　　金：国家攀登计划资助项目(试启动课题)~~

摘　　要：背景:安宫牛黄丸因含有汞、砷成分,其安全性受到关注,有必要进行有效性和安全性评价研究。目的:研究生理、病理状态下安宫牛黄丸对机体作用的差异。设计:以实验动物为研究对象的随机对照实验。单位:一所大学的临床药理研究所。材料:实验于2001-03/04在广州中医药大学临床药理研究所完成,广东省医学实验动物中心提供体质量250～300gSD雄性大鼠24只。方法:SD大鼠随机分成4组:正常组;正常+安宫牛黄丸组;脑梗死模型组(光化学诱导大鼠大脑中动脉闭塞);脑梗死模型+安宫牛黄丸组。每组6只。给药方法:每天胃饲1次,0.13g/kg,共7d。主要观察指标:血清乳酸脱氢酶同工酶LDH1～5含量。结果:正常+安宫牛黄丸组大鼠血清LDH1～3含量比正常组显著升高(P<0.01),其中正常+安宫牛黄丸组LDH1,LDH2,LDH3的值分别为(17.02±0.46),(14.70±0.18),(15.47±0.13)%,正常组则为(11.25±0.70),(8.26±0.90),(12.86±0.90)%;模型+安宫牛黄丸组大鼠血清LDH3(15.51±2.60,%)比模型组(10.93±2.10,%)显著升高(P<0.01),LDH4含量显著降低,其值分别为(22.62±3.00)%,(28.18±0.80)%(P<0.01)。结论:在局灶性脑梗死病理状态下安宫牛黄丸对机体的损伤作用比在正常生理状态下小,提示安宫牛黄丸在生理、病理状态下,对机体的作用方式存在差异。BACKGROUND:Angong niuhuang wan consists of mercury and arsenic,which has draw n the attention on its safety.It is necessary to carry on the research on evalua tion in its availability and safety.OBJECTIVE:To study the mechanic differences of angong niuhuang wan in the org anic body physiologically and pathologically.DESIGN:Randomized controlled experiment based on the experimental animals.SETTING:Institute of clinical pharmacology affiliated to a university.MATERIALS: The experiment was performed in Institute of Clinical Pharmacology affiliated to Guangzhou University of Traditional Chinese Medicine from March t o April in 2001.Guangdong Medical Experimental Animal Center provided 24 SD male rats, weighted varied from 250 g to 300 g.METHODS:SD rats were randomized into 4 groups,named as normal group,normal & angong niuhuang wan group(normal & wan group),model of cerebral infarction g roup(model group)(The middle cerebral artery embolism was induced in rats by pho tochemistry.) and model of cerebral infarction group & angong niuhuang wan gro up (model & wan group),6 rats in each group.Medication instruction: gastric fe eding was applied once daily, 0.13 g/kg,totally for 7 days.MAIN OUTCOME MEASURES:contents of lactate dehydrogenase (LDH) isoenzymes,LDH1 - 5.RESULTS:Contents of serum LDH1- 3 in normal & wan group were significantly increased than those in normal group(P< 0.01),of which, the values of LDH1,LDH2 ,LDH3 were(17.02± 0.46)% , (14.70± 0.18)% ,(15.47± 0.13)% successively in normal & wan group,were(11.25 ± 0.70)% ,(8.26± 0.90)% ,(12.86± 0.9 0)% successively in normal group.Content of serum LDH3[(15.51± 2.60)% [ in m odel & wan group was significantly increased compared with that in model group [(10.93± 2.10)% ](P< 0.01). The contents of LDH4 were[(22.62 ± 3.00)% ] and(28.18± 0.80)% respectively in two groups, indicating significantly red ucing(P< 0.01).CONCLUSION:The organic injury of angong niuhuang wan is less in pathological state of focal cerebral infarction than

关 键 词：安宫牛黄丸 乳酸脱氢酶同工酶类 脑梗死 

分 类 号：R743[医药卫生—神经病学与精神病学]